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01-09-2020 | Metoprolol | Original Contribution

Macrophage-mediated regulation of catecholamines in sympathetic neural remodeling after myocardial infarction

Authors: Juanjuan Lyu, Man Wang, Xinjiang Kang, Huadong Xu, Zhongming Cao, Tao Yu, Keli Huang, Jin Wu, Xinchuan Wei, Qian Lei

Published in: Basic Research in Cardiology | Issue 5/2020

Abstract

Sympathetic neural remodeling, which involves the inflammatory response, plays an important role in ventricular arrhythmias (VAs) after myocardial infarction (MI). Adrenergic receptors on macrophages potentially modulate the inflammatory response. We hypothesized that the increased level of catecholamines activates macrophages and regulates sympathetic neural remodeling after MI. We treated MI mice with either clodronate or metoprolol for 5 days following coronary artery ligation. Mice without treatment after MI and sham-operation mice served as the positive control and negative control, respectively. The norepinephrine levels in plasma and the peri-infarct myocardium increased by almost two-fold in the MI mice compared with the sham-operation mice. Both in vivo and ex vivo electrophysiology examinations showed that the vulnerability to VAs induced by MI was alleviated by macrophage depletion with clodronate and β1-adrenergic blockade with metoprolol, which was in line with circulating and peri-infarct norepinephrine levels, sympathetic reinnervation, and the expression of nerve growth factor (NGF) 7 days after surgery. To further verify the interaction between catecholamines and macrophages, we preconditioned lipopolysaccharide-stimulated RAW 264.7 cells using epinephrine or epinephrine with selective adrenergic antagonists. The expression and release of inflammatory factors including NGF were enhanced by epinephrine. This effect was inhibited by metoprolol but not by other subtype antagonists. Our data suggested that the increased level of catecholamines, traditionally known as positive inotropes secreted from sympathetic nerve endings, might regulate cardiac sympathetic neural remodeling through β1-adrenergic receptors on macrophages, subsequently inducing VAs after MI.

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Title: Macrophage-mediated regulation of catecholamines in sympathetic neural remodeling after myocardial infarction
Authors: Juanjuan Lyu
Man Wang
Xinjiang Kang
Huadong Xu
Zhongming Cao
Tao Yu
Keli Huang
Jin Wu
Xinchuan Wei
Qian Lei
Publication date: 01-09-2020
Publisher: Springer Berlin Heidelberg
Keywords: Metoprolol
Myocardial Infarction
Published in: Basic Research in Cardiology / Issue 5/2020
Print ISSN: 0300-8428
Electronic ISSN: 1435-1803
DOI: https://doi.org/10.1007/s00395-020-0813-3

2024 ASCO Annual Meeting

Springer Medicine

Macrophage-mediated regulation of catecholamines in sympathetic neural remodeling after myocardial infarction

Abstract

2024 ASCO Annual Meeting

Springer Medicine

Abstract

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