Top

International Journal of Colorectal Disease

Published in:

01-11-2009 | Original Article

Gene expressions of HMGI-C and HMGI(Y) are associated with stage and metastasis in colorectal cancer

Authors: Meng-Lin Huang, Chou-Chan Chen, Li-Ching Chang

Published in: International Journal of Colorectal Disease | Issue 11/2009

Abstract

Purpose

The high mobility group proteins (HMGs) include the HMGI family members HMGI-C and HMGI(Y), whose expressions in adult tissues generally correlate with malignant tumor phenotypes. The aim of this study was to assess the relationship of HMGI-C or HMGI(Y) gene expression and prognosis in colorectal cancer patients.

Methods

The gene expressions of HMGI-C and HMGI(Y) in 31 paired samples of colorectal tumor and corresponding non-tumor were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR).

Results

The expression of HMGI(Y) in a colorectal cancer tumor was associated with Dukes staging (p = 0.044), while, in non-tumor, the expression of this gene was significant with metastasis (p = 0.003). Patients with Dukes stage A and B present high HMGI(Y) expression in non-tumor of colorectal cancer (p = 0.006). However, patients with Dukes stage C and D present high HMGI-C expression in colorectal tumor (p = 0.023). In the non-metastasis group, HMGI(Y) was highly expressed in non-tumor of colorectal cancer. However, in the metastasis group, there was no significant difference between tumor and non-tumor tissues in both HMGI-C and HMGI(Y) gene expressions.

Conclusions

The HMGI-C and HMGI(Y) quantitations by real-time RT-PCR are associated with Dukes staging and metastasis; hence, the gene expression levels may be useful in clinical practice.

Reeves R (2000) Structure and function of the HMGI(Y) family of architectural transcription factors. Environ Health Perspect 108:803–809PubMedCrossRef

Friedmann M, Holth LT, Zoghbi HY et al (1993) Organization, inducible-expression and chromosome localization of the human HMG-I(Y) nonhistone protein gene. Nucleic Acids Res 21:4259–4267PubMedCrossRef

Ishwad CS, Shriver MD, Lassige DM et al (1997) The high mobility group I-C gene (HMGI-C): polymorphism and genetic localization. Hum Genet 99:103–105PubMedCrossRef

Tallini G, Dal Cin P (1999) HMGI(Y) and HMGI-C dysregulation: a common occurrence in human tumors. Adv Anat Pathol 6:237–246PubMedCrossRef

Nissen MS, Reeves R (1995) Changes in superhelicity are introduced into closed circular DNA by binding of high mobility group protein I/Y. J Biol Chem 270:4355–4360PubMedCrossRef

Tallini G, Dal Cin P, Rhoden KJ et al (1997) Expression of HMGI-C and HMGI(Y) in ordinary lipoma and atypical lipomatous tumors: immunohistochemical reactivity correlates with karyotypic alterations. Am J Pathol 151:37–43PubMed

Meyer B, Krisponeit D, Junghanss C et al (2007) Quantitative expression analysis in peripheral blood of patients with chronic myeloid leukaemia: correlation between HMGA2 expression and white blood cell count. Leuk Lymphoma 48:2008–2013PubMedCrossRef

Fedele M, Battista S, Kenyon L et al (2002) Overexpression of the HMGA2 gene in transgenic mice leads to the onset of pituitary adenomas. Oncogene 21:3190–3198PubMedCrossRef

Di Cello F, Hillion J, Hristov A et al (2008) HMGA2 participates in transformation in human lung cancer. Mol Cancer Res 6:743–750PubMedCrossRef

10.

Miyazawa J, Mitoro A, Kawashiri S et al (2004) Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity. Cancer Res 64:2024–2029PubMedCrossRef

11.

Battista S, de Nigris F, Fedele M et al (1998) Increase in AP-1 activity is a general event in thyroid cell transformation in vitro and in vivo. Oncogene 17:377–385PubMedCrossRef

12.

Li D, Lin HH, McMahon M et al (1997) Oncogenic raf-1 induces the expression of non-histone chromosomal architectural protein HMGI-C via a p44/p42 mitogen-activated protein kinase-dependent pathway in salivary epithelial cells. J Biol Chem 272:25062–25070PubMedCrossRef

13.

Vallone D, Battista S, Pierantoni GM et al (1997) Neoplastic transformation of rat thyroid cells requires the junB and fra-1 gene induction which is dependent on the HMGI-C gene product. EMBO J 16:5310–5321PubMedCrossRef

14.

Boo LM, Lin HH, Chung V et al (2005) High mobility group A2 potentiates genotoxic stress in part through the modulation of basal and DNA damage-dependent phosphatidylinositol 3-kinase-related protein kinase activation. Cancer Res 65:6622–6630PubMedCrossRef

15.

De Martino I, Visone R, Wierinckx A et al (2009) HMGA proteins up-regulate CCNB2 gene in mouse and human pituitary adenomas. Cancer Res 69:1844–1850PubMedCrossRef

16.

De Martino I, Visone R, Palmieri D et al (2007) The Mia/Cd-rap gene expression is downregulated by the high-mobility group A proteins in mouse pituitary adenomas. Endocr Relat Cancer 14:875–886PubMedCrossRef

17.

Tamimi Y, van der Poel HG, Karthaus HFM et al (1996) A retrospective study of high mobility group I(Y) as progressive marker for prostate cancer determined by in situ hybridization. Br J Cancer 74:573–578PubMed

18.

Czyz W, Balcerczak E, Jakubiak M et al (2004) HMGI(Y) gene expression as a potential marker of thyroid follicular carcinoma. Langenbecks Arch Surg 389:193–197PubMedCrossRef

19.

Pierantoni GM, Fedele M, Pentimalli F et al (2001) High mobility group I (Y) proteins bind HIPK2, a serine-threonine kinase protein which inhibits cell growth. Oncogene 20:6132–6141PubMedCrossRef

20.

Bustin M, Reeves R (1996) High mobility group chromosomal proteins: architectural components that facilitate chromatin function. Prog Nucleic Acid Res Mol Biol 54:35–100PubMedCrossRef

21.

Fedele M, Bandiera A, Chiappetta G (1996) Human colorectal carcinomas express high levels of high mobility group HMGI(Y) proteins. Cancer Res 56:1896–1901PubMed

22.

Kim DH, Park YS, Park CJ et al (1999) Expression of the HMGI(Y) gene in human colorectal cancer. Int J Cancer 84:376–380PubMedCrossRef

23.

Abe N, Watanabe T, Sugiyama M et al (1999) Determination of high mobility group I(Y) expression level in colorectal neoplasias: a potential diagnostic marker. Cancer Res 59:1169–1174PubMed

24.

Tomonaga T, Matsushita K, Yamaguchi S et al (2004) Identification of altered protein expression and post-translational modifications in primary colorectal cancer by using agarose two-dimensional gel electrophoresis. Clin Cancer Res 10:2007–2014PubMedCrossRef

25.

Rogalla P, Drechsler K, Frey G (1996) HMGI-C expression patterns in human tissues. Implications for the genesis of frequent mesenchymal tumors. Am J Pathol 149:775–779PubMed

26.

Elton T, Reeves R (1986) Purification and postsynthetic modifications of friend erythroleukemic cell high mobility group protein HMG-“I”. Anal Biochem 157:53–62PubMedCrossRef

27.

Lanahan A, Williams JB, Sanders LK et al (1992) Growth factor-induced delayed early response genes. Mol Cell Biol 12:3919–3929PubMed

28.

Chiappetta G, Tallini G, De Biasio MC et al (1998) Detection of high mobility group I HMGI(Y) protein in the diagnosis of thyroid tumors: HMGI(Y) expression represents a potential diagnostic indicator of carcinoma. Cancer Res 58:4193–4198PubMed

29.

Giancotti V, Bandiera A, Buratti E et al (1991) Comparison of multiple forms of the high mobility group I proteins in rodents and human cells: identification of the human high mobility group I-C protein. Eur J Biochem 198:211–216PubMedCrossRef

30.

Balcerczak M, Pasz-Walczak G, Balcerczak E (2003) HMGI(Y) gene expression in colorectal cancer: comparison with some histological typing, grading, and clinical staging. Pathol Res Pract 199:641–646PubMedCrossRef

31.

Sezer O, Langelotz C, Blohmer JU et al (2000) Detection of HMGI-C in the peripheral blood of breast cancer patients. Eur J Cancer 36:1944–1948PubMedCrossRef

32.

Langelotz C, Schmid P, Jakob C et al (2003) Expression of high-mobility-group-protein HMGI-C mRNA in the peripheral blood is an independent poor prognostic indicator for survival in metastatic breast cancer. Br J Cancer 88:1406–1410PubMedCrossRef

Title: Gene expressions of HMGI-C and HMGI(Y) are associated with stage and metastasis in colorectal cancer
Authors: Meng-Lin Huang
Chou-Chan Chen
Li-Ching Chang
Publication date: 01-11-2009
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 11/2009
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-009-0770-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Gene expressions of HMGI-C and HMGI(Y) are associated with stage and metastasis in colorectal cancer

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 11/2009

Comparison of a colonic J-pouch and transverse coloplasty pouch in patients with rectal cancer after an ultralow anterior resection using fecoflowmetric profiles

A phase I trial of S-1 with oxaliplatin in patients with relapsed and metastatic colorectal cancer

The impact of family history on the outcome of patients with colorectal cancer in a veterans’ hospital

Colorectal cancer screening in Europe: differences in approach; similar barriers to overcome

Consecutive cecum perforation due to incarcerated diaphragmatic hernia after liver surgery

Level of arterial ligation in total mesorectal excision (TME): an anatomical study