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International Journal of Colorectal Disease

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01-06-2007 | Original Article

The effect of heme oxygenase-1 induction by glutamine on TNBS-induced colitis

The effect of glutamine on TNBS colitis

Authors: Murat Giriş, Yeşim Erbil, Semra Doğru-Abbasoğlu, Burcu Tulumoğlu Yanık, Halil Alış, Vakur Olgaç, Gülçin Aykaç Toker

Published in: International Journal of Colorectal Disease | Issue 6/2007

Abstract

Background

Inflammatory bowel disease is a multifactorial inflammatory disease of the colon and rectum with an unknown etiology. In the present study, we aimed to investigate whether heme oxygenase-1 (HO-1) induction by glutamine could protect colitis-induced damage from oxidative, inflammatory, and apoptotic damage.

Method

The rats were divided into four groups. Group 1 had TNBS colitis alone, group 2 had TNBS-induced colitis and glutamine 1 g/kg/day intragastric gavage for 3 days before TNBS solution administration and 15 days following TNBS solution administration, group 3 had glutamine alone 1 g/kg/day intragastric gavage for 18 days before being killed, and group 4 had isotonic saline solution alone 1 cm³/rat intragastric gavage for 18 days before being killed. Colonic malondialdehyde (MDA) levels, glutathione (GSH) levels, caspase-3 activities, and HO-1 expressions of the killed rats were measured. Nuclear factor kappa B (NF-κB) and HO-1 expression were evaluated by immunohistochemical examination of the colonic tissue.

Result

TNBS-induced colitis significantly increased the colonic MDA levels, caspase-3 activities, and HO-1 expression in comparison to the control group. Glutamine treatment was associated with increased HO-1 expression and GSH levels and decreased MDA levels and caspase-3 activity. Histopathological examination revealed that the intestinal mucosal structure was preserved in the glutamine-treated group. In addition to this, treatment with glutamine significantly increased HO-1 expression and decreased NF-κB expression by immunohistochemistry when compared to the TNBS-induced colitis group.

Conclusion

Glutamine reduced colonic damage in TNBS-induced colitis. The mechanism of the protection associated with glutamine was due to antioxidant, antiapoptotic, anti-inflammatory, and HO-1 induction effects.

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Title: The effect of heme oxygenase-1 induction by glutamine on TNBS-induced colitis
The effect of glutamine on TNBS colitis
Authors: Murat Giriş
Yeşim Erbil
Semra Doğru-Abbasoğlu
Burcu Tulumoğlu Yanık
Halil Alış
Vakur Olgaç
Gülçin Aykaç Toker
Publication date: 01-06-2007
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 6/2007
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-006-0238-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The effect of heme oxygenase-1 induction by glutamine on TNBS-induced colitis

The effect of glutamine on TNBS colitis

Abstract

Background

Method

Result

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Method

Result

Conclusion

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