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Child's Nervous System
Published in: Child's Nervous System 1/2009

01-01-2009 | Original Paper

Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine

Authors: Elvis Terci Valera, Maria Angélica Abdalla de Freitas Cortez, Rosane Gomes de Paula Queiroz, Fabio Morato de Oliveira, María Sol Brassesco, Nada Jabado, Damien Faury, Michael S. Bobola, Hélio Rubens Machado, Carlos Alberto Scrideli, Luiz Gonzaga Tone

Published in: Child's Nervous System | Issue 1/2009

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Abstract

Background

Resistance to drug is a major cause of treatment failure in pediatric brain cancer. The multidrug resistance (MDR) phenotype can be mediated by the superfamily of adenosine triphosphate-binding cassette (ABC) transporters. The dynamics of expression of the MDR genes after exposure to chemotherapy, especially the comparison between pediatric brain tumors of different histology, is poorly described.

Objective

To compare the expression profiles of the multidrug resistance genes ABCB1, ABCC1, and ABCG2 in different neuroepithelial pediatric brain tumor cell lines prior and following short-term culture with vinblastine.

Methods

Immortalized lineages from pilocytic astrocytoma (R286), anaplasic astrocytoma (UW467), glioblastoma (SF188), and medulloblastoma (UW3) were exposed to vinblastine sulphate at different schedules (10 and 60 nM for 24 and 72 h). Relative amounts of mRNA expression were analyzed by real-time quantitative polymerase chain reaction. Protein expression was assessed by immunohistochemistry for ABCB1, ABCC1, and ABCG2.

Results

mRNA expression of ABCB1 increased together with augmenting concentration and time of exposure to vinblastine for R286, UW467, and UW3 cell lines. Interestingly, ABCB1 levels of expression diminished in SF188. Following chemotherapy, mRNA expression of ABCC1 decreased in all cell lines other than glioblastoma. ABCG2 expression was influenced by vinblastine only for UW3. The mRNA levels showed consistent association to protein expression in the selected sets of cell lines analyzed.

Conclusions

The pediatric glioblastoma cell line SF188 shows different pattern of expression of multidrug resistance genes when exposed to vinblastine. These preliminary findings may be useful in determining novel strategies of treatment for neuroepithelial pediatric brain tumors.
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Metadata
Title
Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine
Authors
Elvis Terci Valera
Maria Angélica Abdalla de Freitas Cortez
Rosane Gomes de Paula Queiroz
Fabio Morato de Oliveira
María Sol Brassesco
Nada Jabado
Damien Faury
Michael S. Bobola
Hélio Rubens Machado
Carlos Alberto Scrideli
Luiz Gonzaga Tone
Publication date
01-01-2009
Publisher
Springer-Verlag
Published in
Child's Nervous System / Issue 1/2009
Print ISSN: 0256-7040
Electronic ISSN: 1433-0350
DOI
https://doi.org/10.1007/s00381-008-0740-3

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