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01-11-2012 | Original Paper

Role of vascular endothelial growth factor as a tumour marker in osteosarcoma: a prospective study

Authors: Shishir Rastogi, Ramakant Kumar, Sukesh Rao Sankineani, Kanniraj Marimuthu, Laxman Rijal, Shyam Prakash, Divesh Jalan, Shah Alam Khan, Mehar C. Sharma

Published in: International Orthopaedics | Issue 11/2012

Abstract

Purpose

A prospective study was undertaken to evaluate the diagnostic and prognostic significance of serum levels of vascular endothelial growth factor (VEGF) in patients with primary localised osteosarcoma.

Methods

Serum VEGF levels were measured by an enzyme-linked immunosorbent assay (ELISA) in blood samples collected prechemotherapy, postchemotherapy, and postsurgery in 40 patients with histologically proven primary osteosarcoma. Comparison was made between serum VEGF level of healthy controls (n = 10) and prechemotherapy patient sera to evaluate its diagnostic potential. Serum VEGF levels of patients with and without metastasis were compared. Immunohistochemical staining was done to establish the correlation between serum and tissue VEGF expression. The Kaplan–Meier curve was used for survival analysis

Results

No significant relationship was observed between serum VEGF levels and age, gender, tumour size, local recurrence or histopathological subtypes of osteosarcoma. We observed significantly raised mean serum VEGF in patient sera compared with healthy controls (p = 0.001). Significant fall in mean serum VEGF level was observed following chemotherapy (p = 0.001). Patients who developed metastases had significantly higher serum VEGF levels compared with the nonmetastatic group (P = 0.001). Serum VEGF levels correlated well with VEGF expression in tissues.

Conclusion

Serum VEGF levels might prove to be of diagnostic, predictive and prognostic value in patients with primary osteosarcoma, although further studies with larger sample size and longer follow-up is needed to support the hypothesis.

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Title: Role of vascular endothelial growth factor as a tumour marker in osteosarcoma: a prospective study
Authors: Shishir Rastogi
Ramakant Kumar
Sukesh Rao Sankineani
Kanniraj Marimuthu
Laxman Rijal
Shyam Prakash
Divesh Jalan
Shah Alam Khan
Mehar C. Sharma
Publication date: 01-11-2012
Publisher: Springer-Verlag
Published in: International Orthopaedics / Issue 11/2012
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-012-1663-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Role of vascular endothelial growth factor as a tumour marker in osteosarcoma: a prospective study

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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