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01-04-2008 | Original Paper

Effects of freezing on the biomechanical and structural properties of human posterior tibial tendons

Authors: Sandro Giannini, Roberto Buda, Francesco Di Caprio, Patrizia Agati, Adriana Bigi, Viviana De Pasquale, Alessandro Ruggeri

Published in: International Orthopaedics | Issue 2/2008

Abstract

This work analyzes the effects of storage by fresh-freezing at −80°C on the histological, structural and biomechanical properties of the human posterior tibial tendon (PTT), used for ACL reconstruction. Twenty-two PTTs were harvested from eleven donors. For each donor one tendon was frozen at −80°C and thawed in physiological solution at 37°C, and the other was tested without freezing (control). Transmission electron microscopy (TEM), differential scanning calorimetry (DSC) and biomechanical analysis were performed. We found the following mean changes in frozen-thawed tendons compared to controls: TEM showed an increase in the mean diameter of collagen fibrils and in fibril non-occupation mean ratio, while the mean number of fibrils decreased; DSC showed a decrease in mean denaturation temperature and denaturation enthalpy. Biomechanical analysis showed a decrease in ultimate load and ultimate stress, an increase in stiffness and a decrease in ultimate strain of tendons. In conclusion fresh-freezing brings about significant changes in the biomechanical and structural properties of the human PTT. A high variability exists in the biophysical properties of tendons among individuals and in the effects of storage on tendons. Therefore, when choosing an allograft tendon, particular care is needed to choose a biomechanically suitable graft.

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Title: Effects of freezing on the biomechanical and structural properties of human posterior tibial tendons
Authors: Sandro Giannini
Roberto Buda
Francesco Di Caprio
Patrizia Agati
Adriana Bigi
Viviana De Pasquale
Alessandro Ruggeri
Publication date: 01-04-2008
Publisher: Springer-Verlag
Published in: International Orthopaedics / Issue 2/2008
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-006-0297-2

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Effects of freezing on the biomechanical and structural properties of human posterior tibial tendons

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