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01-07-2009 | Original Article

Reduced levels of recent thymic emigrants in acute myeloid leukemia patients

Authors: Yangqiu Li, Qingsong Yin, Lijian Yang, Shaohua Chen, Suxia Geng, Xiuli Wu, Liye Zhong, Christian A. Schmidt, Grzegorz K. Przybylski

Published in: Cancer Immunology, Immunotherapy | Issue 7/2009

Abstract

Background

T cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Our previous study showed skewed expression of T cell receptor beta variable region (TRBV) subfamilies and clonal expansion of T cells in leukemia patients. In the present study, in order to further characterize the T cell immunity in acute myeloid leukemia (AML) patients, the level of recent thymic emigrants (RTE) was analyzed.

Materials and methods

Quantitative analysis of signal joint T cell recombination excision circles (δRec-ψJα sjTRECs) was performed in peripheral blood mononuclear cells (PBMCs) by real-time PCR (TaqMan), and the analysis of 23 TRBV-BD1 sjTRECs was performed by semi-nested PCR. Eighty-eight cases with AML were selected for this study; ten AML cases in complete remission (AML-CR) and 38 healthy individuals served as controls.

Results

The levels of δRec-ψJα sjTRECs in PBMCs and CD3+ T cells were significantly decreased in AML patients, compared with healthy individuals and in patients in completive remission. Also the frequency of 23 TRBV-BD1 sjTRECs, and the number of detectable TRBV subfamily sjTRECs were significantly lower in AML patients than in healthy individuals. Moreover, the sjTRECs numbers and the frequency of TRBV-BD1 sjTRECs showed a progressive linear decline with age in AML patients.

Conclusions

The decreased numbers of universal (δRec-ψJα) and family-specific (TRBV-BD1) sjTRECs indicate that the severe T cell immunodeficiency in AML patients is associated with reduced levels of recent thymic emigrants. In patients achieving complete remission both sjTREC counts return to normal values indicating the recovery of thymic function. Better understanding of the mechanisms underlying persistent immunodeficiency in leukemia patients may lead to novel treatment strategies to enhance immune competence.

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Title: Reduced levels of recent thymic emigrants in acute myeloid leukemia patients
Authors: Yangqiu Li
Qingsong Yin
Lijian Yang
Shaohua Chen
Suxia Geng
Xiuli Wu
Liye Zhong
Christian A. Schmidt
Grzegorz K. Przybylski
Publication date: 01-07-2009
Publisher: Springer-Verlag
Published in: Cancer Immunology, Immunotherapy / Issue 7/2009
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-008-0621-3

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Abstract

Background

Materials and methods

Results

Conclusions

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