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01-10-2007 | Original Article

Single administration of low dose cyclophosphamide augments the antitumor effect of dendritic cell vaccine

Authors: Ji-Yan Liu, Yang Wu, Xiao-Shi Zhang, Jin-Liang Yang, Hong-Li Li, Yong-Qiu Mao, Yi Wang, Xia Cheng, Yong-Qiang Li, Jian-Chuan Xia, Maria Masucci, Yi-Xin Zeng

Published in: Cancer Immunology, Immunotherapy | Issue 10/2007

Abstract

Single administration of low dose cyclophosphamide (CTX) was previously reported to enhance the antitumor efficacy of immunotherapies. To investigate the possible mechanisms for this effect, we examined whether a single administration of low dose CTX could augment the immunogenicity of dendritic cell (DC) vaccines. Fifty milligrams per kilogram body weight dose of CTX was administrated intraperitoneally to mice after B16 melanoma or C26 colon carcinoma tumor models were established, DC vaccine generated from mouse bone marrow and pulsed with B16 or C26 tumor cells lysates were vaccinated 4 days later. CTX treatment potentiated the antitumor effects of the DC vaccine, and increased the proportion of IFN-γ secreting lymphocytes in spleens. Furthermore, a significantly reduced proportion of CD4+CD25+FoxP3+ regulatory T (T_reg) cells was detected by flow cytometry in spleen lymphocytes from tumor-bearing mice treated with CTX. Thus, a single administration of low dose CTX could augment antitumor immune responses of DC vaccine by reducing the proportion of CD4+CD25+FoxP3+ T_reg cells in tumor-bearing mice. Our results suggested a possible mechanism of CTX-induced immunopotentiation and provided a strategy of immunotherapy combining a low dose CTX with DC vaccine.

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Title: Single administration of low dose cyclophosphamide augments the antitumor effect of dendritic cell vaccine
Authors: Ji-Yan Liu
Yang Wu
Xiao-Shi Zhang
Jin-Liang Yang
Hong-Li Li
Yong-Qiu Mao
Yi Wang
Xia Cheng
Yong-Qiang Li
Jian-Chuan Xia
Maria Masucci
Yi-Xin Zeng
Publication date: 01-10-2007
Publisher: Springer-Verlag
Published in: Cancer Immunology, Immunotherapy / Issue 10/2007
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-007-0305-4

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