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01-07-2006 | Original Article

Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes

Authors: Monika Baj-Krzyworzeka, Rafał Szatanek, Kazimierz Węglarczyk, Jarosław Baran, Barbara Urbanowicz, Piotr Brański, Mariusz Z. Ratajczak, Marek Zembala

Published in: Cancer Immunology, Immunotherapy | Issue 7/2006

Abstract

This study was designed to determine the characteristics of tumour cell-derived microvesicles (TMV) and their interactions with human monocytes. TMV were shed spontaneously by three different human cancer cell lines but their release was significantly increased upon activation of the cells with phorbol 12-myristate 13-acetate (PMA). TMV showed the presence of several surface determinants of tumour cells, e.g. HLA class I, CD29, CD44v7/8, CD51, chemokine receptors (CCR6, CX3CR1), extracellular matrix metalloproteinase inducer (EMMPRIN), epithelial cell adhesion molecule (EpCAM), but their level of expression differed from that on cells they originated from. TMV also carried mRNA for growth factors: vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), interleukin-8 (IL-8) and surface determinants (CD44H). TMV were localized at the monocytes surface following their short exposure to TMV, while at later times intracellularly. TMV transferred CCR6 and CD44v7/8 to monocytes, exerted antiapoptotic effect on monocytes and activated AKT kinase (Protein Kinase B). Thus, TMV interact with monocytes, alter their immunophenotype and biological activity. This implicates the novel mechanism by which tumour infiltrating macrophages may be affected by tumour cells not only by a direct cell to cell contact, soluble factors but also by TMV.

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Title: Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes
Authors: Monika Baj-Krzyworzeka
Rafał Szatanek
Kazimierz Węglarczyk
Jarosław Baran
Barbara Urbanowicz
Piotr Brański
Mariusz Z. Ratajczak
Marek Zembala
Publication date: 01-07-2006
Publisher: Springer-Verlag
Published in: Cancer Immunology, Immunotherapy / Issue 7/2006
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-005-0075-9

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