The present case was a 62-year-old man who presented with relapsing severe colonic diverticular bleeding. A diverticulum with fresh coagula was identified in the ascending colon by colonoscopy and was closed with hemoclips. However, re-bleeding occurred the next day. He underwent emergent enhanced CT, but extravasation was not evident. Gastrointestinal bleeding scintigraphy using 99mTc-human serum albumin diethylenetriaminepentaacetic acid (HSA-DTPA) was performed. Planar images of the anterior abdomen with 99mTc-HSA-DTPA demonstrated faint radioactivity in the area below the right kidney 50 min after injection (a), distinct bowel radioactivity 70 min after injection (b) and radioactivity extending to the transverse colon 100 min after injection (c). Fused single photon emission computed tomography (SPECT)/CT transaxial (d) and coronal (e) images obtained 80 min after injection were able to localize radioactivity in the ascending colon.