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European Journal of Nuclear Medicine and Molecular Imaging

Published in:

01-07-2011 | Original Article

Positron emission tomography imaging of CD105 expression during tumor angiogenesis

Authors: Hao Hong, Yunan Yang, Yin Zhang, Jonathan W. Engle, Todd E. Barnhart, Robert J. Nickles, Bryan R. Leigh, Weibo Cai

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 7/2011

Abstract

Purpose

Overexpression of CD105 (endoglin) correlates with poor prognosis in many solid tumor types. Tumor microvessel density (MVD) assessed by CD105 staining is the current gold standard for evaluating tumor angiogenesis in the clinic. The goal of this study was to develop a positron emission tomography (PET) tracer for imaging CD105 expression.

Methods

TRC105, a chimeric anti-CD105 monoclonal antibody, was conjugated to 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) and labeled with ⁶⁴Cu. FACS analysis and microscopy studies were performed to compare the CD105 binding affinity of TRC105 and DOTA-TRC105. PET imaging, biodistribution, blocking, and ex vivo histology studies were performed on 4T1 murine breast tumor-bearing mice to evaluate the ability of ⁶⁴Cu-DOTA-TRC105 to target tumor angiogenesis. Another chimeric antibody, cetuximab, was used as an isotype-matched control.

Results

FACS analysis of human umbilical vein endothelial cells (HUVECs) revealed no difference in CD105 binding affinity between TRC105 and DOTA-TRC105, which was further validated by fluorescence microscopy. ⁶⁴Cu labeling was achieved with high yield and specific activity. Serial PET imaging revealed that the 4T1 tumor uptake of the tracer was 8.0 ± 0.5, 10.4 ± 2.8, and 9.7 ± 1.8%ID/g at 4, 24, and 48 h post-injection, respectively (n = 3), higher than most organs at late time points which provided excellent tumor contrast. Biodistribution data as measured by gamma counting were consistent with the PET findings. Blocking experiments, control studies with ⁶⁴Cu-DOTA-cetuximab, as well as ex vivo histology all confirmed the in vivo target specificity of ⁶⁴Cu-DOTA-TRC105.

Conclusion

This is the first successful PET imaging study of CD105 expression. Fast, prominent, persistent, and CD105-specific uptake of the tracer in the 4T1 tumor was observed. Further studies are warranted and currently underway.

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Title: Positron emission tomography imaging of CD105 expression during tumor angiogenesis
Authors: Hao Hong
Yunan Yang
Yin Zhang
Jonathan W. Engle
Todd E. Barnhart
Robert J. Nickles
Bryan R. Leigh
Weibo Cai
Publication date: 01-07-2011
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue 7/2011
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-011-1765-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Positron emission tomography imaging of CD105 expression during tumor angiogenesis

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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