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European Journal of Nuclear Medicine and Molecular Imaging

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Open Access 01-04-2010 | Guidelines

Guidelines for the labelling of leucocytes with ¹¹¹In-oxine

Authors: Manel Roca, Erik F. J. de Vries, Francois Jamar, Ora Israel, Alberto Signore

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 4/2010

Abstract

We describe here a protocol for labelling autologous white blood cells with ¹¹¹In-oxine based on previously published consensus papers and guidelines. This protocol includes quality control and safety procedures and is in accordance with current European Union regulations and International Atomic Energy Agency recommendations.

Guidelines on current good radiopharmacy practice (cGRPP) in the preparation of radiopharmaceuticals. EANM Radiopharmacy committee. Eur J Nucl Med Mol Imaging 2009 (in press), https://www.eanm.org/scientific_info/guidelines/gl_radioph_cgrpp.pdf

Becker W. Guidelines for indium-111-oxine leukocyte scintigraphy in inflammatory or infectious diseases. Nuklearmedizin 1999;38:244–6.PubMed

Danpure HJ, Osman S. A review of methods of separating and radiolabelling human leucocytes. Nucl Med Commun 1988;9:681–5.PubMedCrossRef

Datz FL. Indium-111-labeled leukocytes for the detection of infection: current status. Semin Nucl Med 1994;24:92–109.CrossRefPubMed

Dutton JA, Bird NJ, Skehan SJ, Peters AM. Evaluation of a 3-hour indium-111 leukocyte image as a surrogate for a technetium-99m nanocolloid marrow scan in the diagnosis of orthopedic infection. Clin Nucl Med 2004;29:469–74.CrossRefPubMed

Ivancevic V, Munz DL. Nuclear medicine imaging of endocarditis. Q J Nucl Med 1999;43:93–9.PubMed

Joseph TN, Mujtaba M, Chen AL, Maurer SL, Zuckerman JD, Maldjian C, et al. Efficacy of combined technetium-99m sulfur colloid/indium-111 leukocyte scans to detect infected total hip and knee arthroplasties. J Arthroplasty 2001;16:753–8.CrossRefPubMed

Loreal O, Moisan A, Bretagne JF, LeCloirec J, Raoul JL, Herry JY, Gastard J. Scintigraphic assessment of indium-111-labeled granulocyte splenic pooling: a new approach to inflammatory bowel disease activity. J Nucl Med 1990;31:1470–3.PubMed

Martin-Comin J, Prats E. Clinical applications of radiolabeled blood elements in inflammatory bowel disease. Q J Nucl Med 1999;43:74–82.PubMed

10.

Medina M, Viglietti AL, Gozzoli L, Lucano A, Ravasi L, Lucignani G, et al. Indium-111 labelled white blood cell scintigraphy in cranial and spinal septic lesions. Eur J Nucl Med 2000;27:1473–80.CrossRefPubMed

11.

Rennen HJ, Boerman OC, Oyen WJ, Corstens FH. Imaging infection/inflammation in the new millennium. Eur J Nucl Med 2001;28:241–52.CrossRefPubMed

12.

Saverymuttu SH, Peters AM, Dampure HJ, et al. Lung transit of 111Indium-labelled granulocytes. Relationship to labeling technique. Scand J Haematol 1983;30:151–60.PubMed

13.

Seabold JE, Forstrom LA, Schauwecker DS, Brown ML, Datz FL, McAfee JG, et al. Procedure guideline for indium-111-leukocyte scintigraphy for suspected infection/inflammation. J Nucl Med 1997;38:997–1001.PubMed

14.

Segal AW, Arnot RN, Thakur ML, Lavender JP. Indium-111-labelled leucocytes for localisation of abscesses. Lancet 1976;7994:1056–8.CrossRef

15.

Signore A, Beales P, Sensi M, Zuccarini O, Pozzilli P. Labelling of lymphocytes with indium 111 oxine: effect on cell surface phenotype and antibody-dependent cellular cytotoxicity. Immunol Lett 1983;6:151–4.CrossRefPubMed

16.

Signore A, Pozzilli C. Homing and circulation of indium-111-labelled leucocytes. A post-mortem study. Diagn Imaging Clin Med 1985;54:315–7.PubMed

17.

Stoeckli TC, Zimmerli W, Maecke HR, Fridich R. Comparison of chemotaxis and superoxide generation of indium-111-oxine and technetium-99m-HMPAO-labelled granulocytes. Scand J Clin Lab Invest 1996;56:305–9.CrossRefPubMed

18.

Takahashi K, Ohyanagi M, Naruse H, Masai M, Iwasaki T, Fukuchi M, et al. Usefulness of indium-111-oxine-labeled leukocyte scintigraphy in diagnosis of inflammation associated with chronic aortic dissection. Ann Nucl Med 2001;15:527–32.CrossRefPubMed

19.

Teller RE, Christie MJ, Martin W, Nance EP, Haas DW. Sequential indium-labeled leukocyte and bone scans to diagnose prosthetic joint infection. Clin Orthop Relat Res 2000;373:241–7.CrossRefPubMed

20.

Thakur ML, Lavender JP, Arnot RN, Silvester DJ, Segal AW. Indium-111-labeled autologous leukocytes in man. J Nucl Med 1977;18:1014–21.PubMed

21.

Thakur ML, Coleman RE, Welch MJ. Indium-111-labeled leukocytes for the localization of abscesses: preparation, analysis, tissue distribution, and comparison with gallium-67 citrate in dogs. J Lab Clin Med 1977;89:217–28.PubMed

22.

Thomas P, Forstrom L. In-111 labeled purified granulocytes in the diagnosis of synthetic vascular graft infections. Clin Nucl Med 1994;19:1075–8.CrossRefPubMed

23.

Wanahita A, Villeda C, Kutka N, Ramirez J, Musher D. Diagnostic sensitivity and specificity of the radionuclide (indium)-labeled leukocyte scan. J Infect 2007;55:214–9.CrossRefPubMed

24.

Thakur ML. Cell labeling: achievements, challenges and prospects. J Nucl Med 1981;22:1011–4.PubMed

25.

UK Radiopharmacy Group. Guidelines for the safe preparation of radiolabelled blood cells. http://www.ukrg.org.uk/

26.

Yeo C, Saunders N, Locca D, Flett A, Preston M, Brookman P, et al. Ficoll-Paque versus Lymphoprep: a comparative study of two density gradient media for therapeutic bone marrow mononuclear cell preparations. Regen Med 2009;4:689–96.CrossRefPubMed

Title: Guidelines for the labelling of leucocytes with 111In-oxine
Authors: Manel Roca
Erik F. J. de Vries
Francois Jamar
Ora Israel
Alberto Signore
Publication date: 01-04-2010
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue 4/2010
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-010-1393-5

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Guidelines for the labelling of leucocytes with ¹¹¹In-oxine

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