Top

Published in:

01-04-2010 | Original Paper

Orthosiphon grandiflorum has a protective effect in a calcium oxalate stone forming rat model

Authors: Wongsawat Akanae, Masao Tsujihata, Iwao Yoshioka, Norio Nonomura, Akihiko Okuyama

Published in: Urolithiasis | Issue 2/2010

Abstract

This study amied to investigate the effects of Orthosiphon grandiflorum on the renal tubular cell injury induced by oxalate and the inhibitory effects of O. grandiflorum on urinary deposit formation in an animal model and compared the results with those from a potassium citrate treatment. Rats were divided into three groups: an untreated stone-forming group, an O. grandiflorum-treated stone-forming group and a potassium citrate-treated stone forming group. Ethylene glycol (0.5%) was administered to the rats during the last week, and vitamin D3 (0.5 μm) was force fed to induce hyperoxaluria and kidney calcium oxalate crystal deposition. Twenty-four hour urine samples were collected before and after inducing crystal deposits. Rats were killed and both kidneys were harvested after 3 weeks. Bisected kidneys were examined under a polarized light microscope to determine the number of crystals. The renal tissue superoxide dismutase and catalase levels were measured by Western blot. Oxidative stress was examined by 8-OHdG immunohistofluorescence. O. grandiflorum and potassium citrate have the ability to alkalinize urine. Among all groups, the number of crystal deposits and the level of 8-OHdG staining decreased significantly in the O. grandiflorum-treated stone forming group, as compared to the other groups. Superoxide dismutase and catalase levels also increased significantly in the O. grandiflorum-treated stone-forming group, as compared with the untreated stone-forming group. The results indicate that O. grandiflorum has a significant inhibitory effect on crystal deposition in the calcium oxalate-stone-forming rat model.

Ramello A, Vitale C, Marangella M (2000) Epidemiology of nephrolithiasis. J Nephrol 3:45–50

Sriboonlue P, Prasongwattana V, Chatta K, Tungsanga K (1992) Prevalence of upper urinary tract stone disease in a rural community of northeast Thailand. Br J Urol 69:240–244CrossRefPubMed

Yanakawa M, Kawamura J, Onishi T et al (1997) Incidence of urolithiasis in northeast Thailand. Int J Urol 4:537–540CrossRef

Coe FL, Evan A, Worcester E (2005) Kidney stone disease. J Clin Invest 115:2598–2608CrossRefPubMed

Unakul S (1961) Urinary stones in Thailand: a statistical survey. Siriraj Hospital Gaz 13:199–214

Prasongwattana V, Sriboonlue P, Suntarapa S (1983) Urinary stone composition in northeast Thailand. Br J Urol 55:353–355CrossRef

Osamu Y, Akito T, Tadashi O, Yusaku O (1999) Nation trend of the incidence of urolithiasis in Japan from 1965 to 1995. Kidney Int 56:1899–1904CrossRef

Pearle MS, Calhoun EA, Curhan GC et al (2005) Urologic diseases in America project: urolithiasis. J Urol 173:848–857CrossRefPubMed

Aeckart KSJ, Schroder FH (1989) Effect of extra corporeal shock wave lithotripsy (ESWL) on renal tissue. Urol Res 17:3–7

10.

Touhami M, Laroubi A, Elhabazi K et al. (2007) Lemon juice has protective activity in a rat urolithiasis model. BMC Urol 5:7–18

11.

Chin JH, Hussin AH, Ismail S (2009) Effect of Orthosiphon Stamineus leaf extracts on hepatic cytochrome P450, UGT and GST activity in STZ-induced diabetic rats. J Adv Sci Arts 1(1)

12.

Lyckender IM, Malterud KE (1996) Lipophillic flavonoids from Orthosiphon Stamineus prevent oxidative inactivation of 15-lipoxygenase. Prostaglandins Leukot Essent Fatty Acids 54(4):239–246CrossRef

13.

Malterud KE, Rydland KM (2000) Inhibition of 15-lipoxygenase from orange peel. J Agri Food Chem 48(11):5576–5580CrossRef

14.

Takeda Y, Matsumoto T, Terao H et al (1993) Orthosiphol D and E minor diterpenes from Orthosiphon Stamineus. Phytochemistry 33(2):411–415CrossRef

15.

Tezuka Y, Stampoulis P, Banskota AH et al (2000) Constituents of the Vietnamese medical plant Orthosiphon Stamineus. Chem Pharm Bull 48(11):1711–1719PubMed

16.

Sumaryono W, Proksch P, Wray V et al (1991) Qualitative and quantitative analysis of the phenolic constituents from Orthosiphon Stamineus. Plant Med 57:176–180CrossRef

17.

RPark HK, Jeong BC, Sung MK, Park MY et al (2008) Reduction of oxidative stress in cultured renal tubular cells and preventive effects on renal stone formation by the bioflavonoid quercetin. J Urol 179(4):1620–1626CrossRef

18.

Jeong BC, Kim BS, Kim JI, Kim HH (2006) Effects of green tea on urinary stone formation: an in vivo and in vitro study. J Endourol 20(5):356–361CrossRefPubMed

19.

Kalidas S, Gopinadhan P, Anthony P, Robert EL (2006) 2.11 Rosmarinic acid biosynthesis and mechanism of action. In: Food biotechnology 2nd edn. Mortimer, London

20.

Premgamone A, Sriboonlue P, Disatapornjaroen W et al (2001) A long-term study on the efficacy of a herbal plant, Orthosiphon grandiflorus, and sodium potassium citrate in renal calculi treatment. Southeast Asian J Trop Med Public Health 32(3):654–660PubMed

21.

Robinson MR, Leitao VA, Haleblian GE, Scales CD Jr, Chandrashekar A, Pierre SA, Preminger GM (2009) Impact of long-term potassium citrate therapy on urinary profiles and recurrence stone formation. J Urol 181(3):1145–1150CrossRefPubMed

22.

Ogawa Y, Machida N, Ogawa T et al (2006) Calcium oxalate supersaturation in dialysis patients with and without primary hyperoxaluria. Urol Res 34(1):12–16CrossRefPubMed

23.

Luke MN (2007) Quantifying western blots without expensive commercial quantification software. In: Miscellaneous topics all vaguely related to science. http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html. Accessed 01 August 2007

24.

Churchill DN (1987) Medical treatment to prevent recurrent calcium urolithiasis: a guide to critical appraisal. Miner Electrolyte Metab 13:294–304PubMed

25.

Preminger GM (1992) Renal-calculi: pathogenesis, diagnosis, and medical therapy. Semin Nephrol 12:200–216PubMed

26.

Mattle D, Hess B (2005) Preventive treatment of neprolithiasis with alkali citrate: a critical review. Urol Res 33(2):73–79CrossRefPubMed

27.

Preminger GM, Sakhaee K, Pak CY (1988) Alkali action on the urinary crystallization of calcium salts: contrasting responses to sodium citrate and potassium citrate. J Urol 139(2):240–242PubMed

28.

Barcelo P, Wuhl O, Servitge E, Rousaud A, Park CY (1993) Randomized double-blind study of potassium citrate in idiopathic hypocitrauric calcium nephrolithiasis. J Urol 150(6):1761–1764PubMed

29.

Stevenson AE, Robertson WG, Markwell P (2003) Risk factor analysis and relative supersaturation as tools for identifying calcium oxalate stone-forming dogs. J Small Anim Pract 44(11):491–496CrossRefPubMed

30.

Carvalho M, Vieira MA (2004) Changes in calcium oxalate crystal morphology as a function of supersaturation. Int Braz J Urol 30(3):205–209CrossRefPubMed

31.

Miller C, Kennington L, Cooney R et al (2000) Oxalate toxicity in renal epithelial cells: characteristics of apoptosis and necrosis. Toxicol Appl Pharmacol 162:132–141CrossRefPubMed

32.

Thamilselvan S, Khan SR (1998) Oxalate and calcium oxalate crystals are injurious to renal epithelial cells: results of in vivo and in vitro studies. J Nephrol 11(1):66–69PubMed

33.

Khan SR, Byer KJ, Thamilselvan S et al (1999) Crystal-cell interaction and apoptosis in oxalate-associated injury of renal epithelial cells. J Am Soc Nephrol 10(14):457–463

34.

Tsujihata M, Momohara C, Yoshioka I, Tsujimura A, Nonomura N, Okuyama A (2008) Atorvastatin inhibits renal crystal retention in a rat stone forming model. J Urol 180(5):2212–2217CrossRefPubMed

35.

Schriner SE, Ogburn CE, Smith AC et al (2000) Level of DNA damage are unaltered in mice overexpressing human catalase in nuclei. Free Radic Biol Med 29(7):664–673CrossRefPubMed

36.

Calişkan-Can E, Firat H, Ardiç S, Simşek B, Torun M, Yardim-Akaydin S (2008) Increased levels of 8-hydroxydeoxyguanosine and its relationship with lipid peroxidation and antioxidant vitamins in lung cancer. Clin Chem Lab Med 46(1):107–112CrossRefPubMed

37.

Nijveldt RJ, van Nood E, van Hoorn DE et al (2001) Flavonoids: a review of probable mechanism of action and potential applications. Am J Clin Nutr 74(4):418–425PubMed

38.

Itoh Y, Yasui T, Okada A et al (2005) Preventive effects of green tea on renal stone formation and the role of oxidative stress in nephrolithiasis. J Urol 173(1):271–275PubMedCrossRef

39.

Khan SR (2005) Hyperoxaluria-induced oxidative stress and antioxidants for renal protection. Urol Res 33(5):349–357CrossRefPubMed

Title: Orthosiphon grandiflorum has a protective effect in a calcium oxalate stone forming rat model
Authors: Wongsawat Akanae
Masao Tsujihata
Iwao Yoshioka
Norio Nonomura
Akihiko Okuyama
Publication date: 01-04-2010
Publisher: Springer-Verlag
Published in: Urolithiasis / Issue 2/2010
Print ISSN: 2194-7228
Electronic ISSN: 2194-7236
DOI: https://doi.org/10.1007/s00240-010-0265-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Orthosiphon grandiflorum has a protective effect in a calcium oxalate stone forming rat model

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2010

Complications and outcomes following extracorporeal shock wave lithotripsy: a prospective study of 3,241 patients

Urinary chemokines/cytokines are elevated in patients with urolithiasis

Family history in stone disease: how important is it for the onset of the disease and the incidence of recurrence?

The mechanism of renal stone formation and renal failure induced by administration of melamine and cyanuric acid

Induction of apoptosis with cisplatin enhances calcium oxalate crystal adherence to inner medullary collecting duct cells

Antiapoptotic effect of angiotensin-II type-1 receptor blockade in renal tubular cells of hyperoxaluric rats