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Calcified Tissue International
Published in: Calcified Tissue International 2/2011

01-02-2011

Assessment of the Impact of Two Different Isolation Methods on the Osteo/Odontogenic Differentiation Potential of Human Dental Stem Cells Derived from Deciduous Teeth

Authors: Athina Bakopoulou, Gabriele Leyhausen, Joachim Volk, Asterios Tsiftsoglou, Pavlos Garefis, Petros Koidis, Werner Geurtsen

Published in: Calcified Tissue International | Issue 2/2011

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Abstract

Human deciduous teeth have been proposed as a promising source of mesenchymal stem cells for application in bone and dental tissue engineering. We established cultures of mesenchymal stem cells from the pulp of human deciduous teeth (deciduous teeth stem cells, DTSCs) and analyzed their morphologic, growth, immunophenotypic, and osteo/odontogenic differentiation characteristics using different isolation methods and culturing environments. We compared the biologic behavior of DTSCs isolated either by enzymatic dissociation (DTSCs-ED) or by direct outgrowth from pulp tissue explants (DTSCs-OG). We found that different isolation methods give rise to different populations/lineages of cells with respect to their phenotypic and differentiation characteristics. DTSCs-ED cultures comprised heterogeneous cell populations, whereas DTSCs-OG comprised more homogenous spindle-shaped cells. We have characterized DTSCs as STRO-1+/CD146+/CD34+/CD45− cells. However, the percentage of STRO-1+ and CD34+ cells was higher in DTSCs-ED (STRO-1, 17.01 ± 5.04%; CD34, 19.79 ± 4.66%) compared to DTSCs-OG cultures (STRO-1, 5.18 ± 2.39%; CD34, 9.94 ± 3.41%), probably as a result of a higher release of stem/progenitor cells from the perivascular niche during enzymatic dissociation. DTSCs isolated using either method displayed an active potential for cellular migration and biomineralization, giving rise to 3D mineralized structures when challenged with dexamethasone, monopotassium phosphate, and β-glycerophosphate. These cellular aggregates progressively expressed differentiation markers of functional odontoblasts, including dentin sialophosphoprotein, bone sialoprotein, osteocalcin, and alkaline phosphatase, having the characteristics of osteodentin. However, in DTSCs-ED, the mineralization rate and the amount of mineralized matrix produced was higher compared to DTSCs-OG cultures. Therefore, DTSCs-ED cells display enhanced biomineralization potential, which might be of advantage for application in clinical therapy.
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Metadata
Title
Assessment of the Impact of Two Different Isolation Methods on the Osteo/Odontogenic Differentiation Potential of Human Dental Stem Cells Derived from Deciduous Teeth
Authors
Athina Bakopoulou
Gabriele Leyhausen
Joachim Volk
Asterios Tsiftsoglou
Pavlos Garefis
Petros Koidis
Werner Geurtsen
Publication date
01-02-2011
Publisher
Springer-Verlag
Published in
Calcified Tissue International / Issue 2/2011
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-010-9438-0

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