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Published in:

01-04-2010

β₁ Integrins Mediate Mechanosensitive Signaling Pathways in Osteocytes

Authors: Julie B. Litzenberger, Jae-Beom Kim, Padmaja Tummala, Christopher R. Jacobs

Published in: Calcified Tissue International | Issue 4/2010

Abstract

Integrins are cell-substrate adhesion proteins that initiate intracellular signaling and may serve as mechanosensors in bone. MLO-Y4 cells were stably transfected with a dominant negative form of the β₁ integrin subunit (β₁DN) containing the transmembrane domain and cytoplasmic tail of β₁ integrin. Cells expressing β₁DN had reduced vinculin localization to focal contacts but no change in intracellular actin organization. When exposed to oscillatory fluid flow, β₁DN cells exhibited a significant reduction in the upregulation of cyclooxygenase-2 gene expression and prostaglandin E₂ release. Similarly, the ratio of receptor activator of NF-κB ligand mRNA to osteoprotegerin mRNA decreased significantly after exposure to fluid flow in control cells but not in β₁DN cells. Interfering with integrin signaling did not affect mechanically induced intracellular calcium mobilization. These data suggest that integrins may initiate the cellular response of osteocytes to dynamic fluid flow and may serve as mechanosensitive molecules in bone.

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Title: β1 Integrins Mediate Mechanosensitive Signaling Pathways in Osteocytes
Authors: Julie B. Litzenberger
Jae-Beom Kim
Padmaja Tummala
Christopher R. Jacobs
Publication date: 01-04-2010
Publisher: Springer-Verlag
Published in: Calcified Tissue International / Issue 4/2010
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-010-9343-6

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