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Published in:

01-04-2008

Ovariectomy Sensitizes Rat Cortical Bone to Whole-Body Vibration

Authors: Alessandro Rubinacci, Massimo Marenzana, Francesco Cavani, Federica Colasante, Isabella Villa, Johannes Willnecker, Gian Luigi Moro, Luigi Paolo Spreafico, Marzia Ferretti, Francesca Guidobono, Gastone Marotti

Published in: Calcified Tissue International | Issue 4/2008

Abstract

This study was designed to determine the modulatory effect of estrogen on mechanical stimulation in bone. Trabecular and cortical bone compartments of ovariectomized rats exposed to whole-body vibration of different amplitudes were evaluated by peripheral quantitative computed tomographic (pQCT) analysis and histomorphometry and compared to controls not exposed to vibration. Rats underwent whole-body vibration (20 minutes/day, 5 days/week) on a vibration platform for 2 months. The control rats were placed on the platform without vibration for the same time. We divided rats into six groups: a sham control (SHAM); a sham vibrated (SHAM-V) at 30 Hz, 0.6 g; a SHAM-V at 30 Hz, 3g; an ovariectomized control (OVX); an ovariectomized vibrated (OVX-V) at 30 Hz, 0.6 g; and an OVX-V at 30 Hz, 3g. In vivo, pQCT analyses of the tibiae were performed at the start of the experiment and after 4 and 8 weeks. After 8 weeks the tibiae were excised for histomorphometric and for in vitro pQCT analyses. In the SHAM-V group, vibration had no effect upon the different bone parameters. In the OVX-V group, vibration induced a significant increase compared to the OVX group of the cortical and medullary areas (P < 0.01) and of the periosteal (P < 0.01) and endosteal (P < 0.05) perimeters at the 3 g vibration. The strain strength index increased in the OVX-V group significantly (P < 0.01) at the higher vibration. The results showed that low-amplitude, high-frequency whole-body vibration is anabolic to bone in OVX animals. The osteogenic potential is limited to the modeling of the bone cortex and depends on the amplitude of the vibration.

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Title: Ovariectomy Sensitizes Rat Cortical Bone to Whole-Body Vibration
Authors: Alessandro Rubinacci
Massimo Marenzana
Francesco Cavani
Federica Colasante
Isabella Villa
Johannes Willnecker
Gian Luigi Moro
Luigi Paolo Spreafico
Marzia Ferretti
Francesca Guidobono
Gastone Marotti
Publication date: 01-04-2008
Publisher: Springer-Verlag
Published in: Calcified Tissue International / Issue 4/2008
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-008-9115-8

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