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Open Access 01-11-2016 | Original Article

Urinary proteomic pattern in female stress urinary incontinence: a pilot study

Authors: Marianne Koch, Goran Mitulovic, Engelbert Hanzal, Wolfgang Umek, Sonja Seyfert, Thomas Mohr, Heinz Koelbl, Rosa Maria Laterza

Published in: International Urogynecology Journal | Issue 11/2016

Abstract

Introduction and hypothesis

Previous studies aiming to identify specific pre-defined urine protein biomarkers for stress urinary incontinence (SUI) have not identified clinically important differences. The hypothesis of our study was that the global distribution of urinary proteins, the proteome, differs between women with and those without SUI.

Methods

In this age-matched case–control study, we compared the urinary proteome of 20 women with SUI and 20 controls. Proteins were identified by applying high-performance liquid chromatography separation and tandem mass spectrometry detection. Data analysis was performed using Mascot 2.4.1 embedded in ProteinScape 3.1.

Results

We identified 828 different proteins. The concentration of six of those showed a significant difference between urine samples of SUI patients and those of controls (q value < 0.25). Four proteins showed a higher abundance in SUI samples compared with controls: plasma serine protease inhibitor (logFC 1.11), leucine-rich alpha-2-glycoprotein (logFC 3.91), lysosomal alpha-glucosidase (logFC 1.24), and peptidyl-prolyl cis- trans isomerase A (logFC 1.96). We identified two proteins in lower abundance in SUI samples compared with controls: uromodulin (logFC −4.87) and TALPID3 (logFC −1.99).

Conclusions

Overexpression of plasma serine protease inhibitor, leucine-rich alpha-2-glycoprotein, lysosomal alpha-glucosidase, and peptidyl-prolyl cis- trans isomerase A, and lower expression of uromodulin and TALPID3, in urine may be associated with female SUI.

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Title: Urinary proteomic pattern in female stress urinary incontinence: a pilot study
Authors: Marianne Koch
Goran Mitulovic
Engelbert Hanzal
Wolfgang Umek
Sonja Seyfert
Thomas Mohr
Heinz Koelbl
Rosa Maria Laterza
Publication date: 01-11-2016
Publisher: Springer London
Published in: International Urogynecology Journal / Issue 11/2016
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-016-3033-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Urinary proteomic pattern in female stress urinary incontinence: a pilot study

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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