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01-04-2008 | Original Article

Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats

Authors: Diaa E. E. Rizk, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Gaber A. Ramadan, Soha S. Al-Kedrah, Sayel A. Daoud, Mohamed A. Fahim

Published in: International Urogynecology Journal | Issue 4/2008

Abstract

We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n = 24 × 2) received 42 daily intraperitoneal 17-ß estradiol (10 μg/kg), ghrelin (2 μg/kg), both, or vehicle (n = 6 × 4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.

Pfisterer MH, Griffiths DJ, Schaefer W, Resnick NM (2006) The effect of age on lower urinary tract function: a study in women. J Am Geriatr Soc 54:405–412PubMedCrossRef

Pretlove SJ, Radley S, Tooz-Hobson PM, Thompson PJ, Coomarasamy A, Khan KS (2006) Prevalence of anal incontinence according to age and gender: a systematic review and meta-regression analysis. Int Urogynecol J 17:407–417CrossRef

Waetjen LE, Dwyer PL (2006) Estrogen therapy and urinary incontinence: what is the evidence and what do we tell our patients? Int Urogynecol J 17:541–545CrossRef

Dubeau CE (2006) The aging lower urinary tract. J Urol 175(Suppl 1):S11–S15PubMedCrossRef

Hendrix SL, Cochrane BB, Nygaard IE, Handa VL, Barnabei VM, Iglesia C et al (2005) Effects of estrogen with and without progestin on urinary incontinence. JAMA 293:935–948PubMedCrossRef

Steinauer JE, Waetjen LE, Vittinghoff E, Subak LL, Hulley SB, Grady D et al, for the Heart and Estrogen/Progestin Replacement Study Research Group (2005) Post-menopausal hormone therapy: does it cause incontinence? Obstet Gynecol 106:940–945PubMed

Rizk DEE, Fahim MA, Hassan HA, Al-Marzouqi AH, Ramadan GA, Al-Kedrah SS et al (2007) The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats. Int Urogynecol J 18:1077–1085CrossRef

Wyman JF, Bliss DZ, Dougherty MC, Gray M, Kaas M, Newman DK et al (2004) Shaping future directions for incontinence research in aging adults: executive summary. Nurs Res 53(Suppl 1):S1–S10PubMedCrossRef

Tritos NA, Kokkotou EG (2006) The physiology and potential clinical applications of ghrelin, a novel peptide hormone. Mayo Clin Proc 81:653–660PubMedCrossRef

10.

Hosoda H, Kojima M, Kangawa K (2006) Biological, physiological, and pharmacological aspects of ghrelin. J Pharmacol Sci 100:398–410PubMedCrossRef

11.

Rizk DEE, Hassan HA, Ramadan GA, Shafiullah M, Fahim MA (2005) Estrogen and ghrelin increase number of submucosal urethral and anal canal blood vessels in ovariectomized rats. Urology 66:1343–1348PubMedCrossRef

12.

Hall R, Kkhalsa S, Qualls C, Rogers RG (2006) A comparison of periurethral blood flow resistive indices and urethral closure pressure of incontinent women. Int Urogynecol J 17:472–477CrossRef

13.

Moalli PA, Talarico LC, Sung VW, Klingensmith WL, Shand SH, Meyn LA et al (2004) Impact of menopause on collagen subtypes in the arcus tendinous fascia pelvis. Am J Obstet Gynecol 190:620–627PubMedCrossRef

14.

Rizk DEE, Mensah-Brown EP, Chandranath SI, Ahmed I, Shafiullah M, Patel M et al (2003) Effects of ovariectomy and hormone replacement on collagen and blood vessels of the urethral submucosa of rats. Urol Res 31:147–151PubMedCrossRef

15.

Mensah-Brown EP, Rizk DEE, Patel M, Chandranath SI, Adem A (2004) Effects of ovariectomy and hormone replacement on submucosal collagen and blood vessels of the anal canal of rats. Colorectal Dis 6:481–487PubMedCrossRef

16.

Camina JP (2006) Cell biology of the ghrelin receptor. J Neuroendocrinol 18:65–76PubMedCrossRef

17.

Gil-Campos M, Aguilera CM, Canete R, Gil A (2006) Ghrelin: a hormone regulating food intake and energy homeostasis. Br J Nutr 96:201–226PubMedCrossRef

18.

Diano S, Farr SA, Benoit SC, McNay EC, da Silva I, Horvath B et al (2006) Ghrelin controls hippocampal spine synapse density and memory performance. Nat Neurosc 9:381–388CrossRef

19.

Li A, Cheng G, Zhu GH, Tarnawski AS (2007) Ghrelin stimulates angiogenesis in human microvascular endothelial cells: implications beyond GH release. Biochem Biophys Res Commun 353:238–243PubMedCrossRef

20.

Fukushima N, Hanada R, Teranishi H, Fukuke Y, Tachibana T, Ishikawa H (2005) Ghrelin directly regulates bone formation. J Bone Miner Res 20:790–798PubMedCrossRef

21.

Kojima M, Kangawa K (2006) Drug insight: the functions of ghrelin and its potential as a multitherapeutic hormone. Nat Clin Pract Endocrinol Metab 2:80–88PubMedCrossRef

22.

Lelbach A, Feher J, Szekacs B (2006) The molecular biology of ageing-therapeutic interventions? Orv Hetil 147:441–448PubMed

23.

Rizk DEE, Al-Marzouqi AH, Al-Kedrah SS, Hassan HA, Fahim MA (2007) Estrogen and ghrelin decrease cytoplasmic expression of p27^kip1, a cellular marker of ageing, in the striated anal sphincter and levator muscle of ovariectomized rats. Int Urogynecol J 18:413–418CrossRef

24.

Veldhuis JD, Keenan DM, Iranmanesh A, Mielke K, Miles JM, Bowers CY (2006) Estradiol potentiates ghrelin-stimulated pulsatile growth hormone secretion in postmenopausal women. J Clin Endocrinol Metab 91:3559–3565PubMedCrossRef

25.

Budak E, Fernandez Sanchez M, Bellver J, Cervero A, Simon C, Pellicer A (2006) Interactions of the hormones leptin, ghrelin, adiponectin, resistin, and PYY3-36 with the reproductive system. Fertil Steril 85:1563–1581PubMedCrossRef

26.

Krause EG, Curtis KS, Stincic TL, Markle JP, Contreras RJ (2006) Oestrogen and weight loss decrease isoproterenol-induced Fos immunoreactivity and angiotensin type 1 mRNA in the subfornical organ of female rats. J Physiol 573:251–262PubMedCrossRef

27.

Altun M, Bergman E, Edstrom E, Johnson H, Ulfhake B (2007) Behavioral impairments of the aging rat. Physiol Behav, DOI 10.1016/j.physbeh.2007.06.017)

28.

Tschop M, Smiley DL, Heiman ML (2000) Ghrelin induces adiposity in rodents. Nature 407:908–913PubMedCrossRef

Title: Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats
Authors: Diaa E. E. Rizk
Hazem A. Hassan
Ahmed H. Al-Marzouqi
Gaber A. Ramadan
Soha S. Al-Kedrah
Sayel A. Daoud
Mohamed A. Fahim
Publication date: 01-04-2008
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 4/2008
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-007-0462-1

Keynote webinar | Spotlight on medication adherence

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Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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