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Knee Surgery, Sports Traumatology, Arthroscopy

Published in:

01-07-2013 | Shoulder

Increased levels of apoptosis and p53 in partial-thickness supraspinatus tendon tears

Authors: Kirsten Lundgreen, Øystein Lian, Alex Scott, Lars Engebretsen

Published in: Knee Surgery, Sports Traumatology, Arthroscopy | Issue 7/2013

Abstract

Purpose

The role of apoptosis in the progression of rotator cuff tendinopathy remains poorly understood. In particular, the extent of apoptosis in the partially torn supraspinatus tendon has not been well examined.

Methods

Biopsies were obtained from nine partially torn supraspinatus tendons, from the matched intact subscapularis tendons, and from 10 reference subscapularis tendons. Immunohistochemistry was used to assess the density of apoptotic cells (activated caspase-3; Asp175), proliferation (Ki67), and p53 (M7001), a key protein involved in regulating cell death. The Bonar scale was used to evaluate tendon degeneration.

Results

The density of apoptotic tendon cells and the density of cells expressing p53 were significantly increased in both the partially torn supraspinatus tendons and in the matched subscapularis tendons, compared with uninjured reference tendons. The Bonar score revealed significant tendon degeneration in the partially torn supraspinatus tendons compared with both matched and reference subscapularis tendons. Tendon cell proliferation was significantly increased in the partially torn supraspinatus tendons compared with reference subscapularis tendons.

Conclusions

Partial-thickness tears of the supraspinatus tendon demonstrated an increased density of apoptotic, p53+ tendon cells. The fact that apoptosis was accompanied by increased tendon cell proliferation suggests that apoptosis may be related to an ongoing injury-repair process. Increased tenocyte apoptosis may be a relatively early feature in rotator cuff tendinopathy and could represent a possible target for therapeutic intervention.

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Title: Increased levels of apoptosis and p53 in partial-thickness supraspinatus tendon tears
Authors: Kirsten Lundgreen
Øystein Lian
Alex Scott
Lars Engebretsen
Publication date: 01-07-2013
Publisher: Springer-Verlag
Published in: Knee Surgery, Sports Traumatology, Arthroscopy / Issue 7/2013
Print ISSN: 0942-2056
Electronic ISSN: 1433-7347
DOI: https://doi.org/10.1007/s00167-012-2226-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Increased levels of apoptosis and p53 in partial-thickness supraspinatus tendon tears

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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