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01-09-2010 | Original

Effects of terbutaline on circulatory failure and organ dysfunction induced by peritonitis in rats

Authors: Cheng-Ming Tsao, Shiu-Jen Chen, Ming-Chung Shih, Wei-Ming Lue, Mei-Yung Tsou, Ann Chen, Wen-Jinn Liaw, Chin-Chen Wu

Published in: Intensive Care Medicine | Issue 9/2010

Abstract

Objective

The pathogenesis of multiple organ dysfunction syndrome (MODS) in septic shock is mainly caused by maldistribution of tissue perfusion and the amplification of inflammatory responses, which may be modulated by β₂-adrenoceptor agonists. We evaluated and compared effects of terbutaline on MODS in a cecal ligation and puncture (CLP) model of sepsis.

Design and setting

Prospective controlled animal study in a university laboratory.

Methods

Male adult Wistar rats received CLP or sham operation followed by the administration of saline or terbutaline (0.3 mg/kg i.v. at 3 and 9 h after CLP).

Measurements and results

At 0, 9 and 18 h after CLP, the changes of hemodynamics, organ function indexes, as well as the plasma interleukin-1β (IL-1β) and nitrite/nitrate levels were examined. At 18 h after CLP, animals were killed and their lungs, livers and kidneys were immediately excised to analyze superoxide anion (O₂ ⁻) levels and inducible nitric oxide synthase (iNOS) expression. These organs were also evaluated by pathological study. Terbutaline significantly (1) prevented delayed hypotension and reduced hepatic and renal dysfunction, (2) diminished plasma IL-1β and nitrite/nitrate, lung iNOS expression, tissue O₂ ⁻ level and the infiltration of neutrophils in the lung and the liver, and (3) improved the 18-h survival rate.

Conclusions

Terbutaline may be developed as an alternative treatment for severe sepsis-induced MODS. The protective effect of terbutaline seems to be through inhibition of proinflammatory mediators and attenuation of oxidant production.

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Title: Effects of terbutaline on circulatory failure and organ dysfunction induced by peritonitis in rats
Authors: Cheng-Ming Tsao
Shiu-Jen Chen
Ming-Chung Shih
Wei-Ming Lue
Mei-Yung Tsou
Ann Chen
Wen-Jinn Liaw
Chin-Chen Wu
Publication date: 01-09-2010
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 9/2010
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-010-1839-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of terbutaline on circulatory failure and organ dysfunction induced by peritonitis in rats

Abstract

Objective

Design and setting

Methods

Measurements and results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Design and setting

Methods

Measurements and results

Conclusions

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