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Intensive Care Medicine

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Open Access 01-04-2009 | Experimental

Endotoxin-induced gene expression differences in the brain and effects of iNOS inhibition and norepinephrine

Authors: Stephanie Wolff, Sabine Klatt, Jens C. Wolff, Jochen Wilhelm, Ludger Fink, Manfred Kaps, Bernhard Rosengarten

Published in: Intensive Care Medicine | Issue 4/2009

Abstract

Purpose

We studied gene expression differences in brain homogenate, hippocampus, somatosensory cortex and cerebellum of rats suffering from sepsis-associated delirium and analyzed the effects of norepinephrine and 1,400 W (specific inhibitor of the inducible nitric-oxide synthase).

Methods

We applied microarray screenings to rat brain homogenate 1, 3 and 4.5 h after lipopolysaccharide (LPS, 5 mg/kg) or 0.9% NaCl treatment. Therapy groups were analyzed after 4.5 h. Validations and compartment specific investigations were carried out by real-time PCR.

Results

Most striking gene expression differences were seen 4.5 h after LPS administration, especially within the hippocampus (chemokines and endothelial cell-specific molecule 1). Norepinephrine resulted in a discrete chemokine up-regulation, while 1,400 W had hardly any effect.

Conclusion

Strongest gene regulations were found within the hippocampus. Norepinephrine showed a tendency of having a proinflammatory influence, while 1,400 W had no clear-cut effect onto the gene expression level.

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Title: Endotoxin-induced gene expression differences in the brain and effects of iNOS inhibition and norepinephrine
Authors: Stephanie Wolff
Sabine Klatt
Jens C. Wolff
Jochen Wilhelm
Ludger Fink
Manfred Kaps
Bernhard Rosengarten
Publication date: 01-04-2009
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 4/2009
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-009-1394-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Endotoxin-induced gene expression differences in the brain and effects of iNOS inhibition and norepinephrine

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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Acknowledgement to reviewers

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