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01-04-2009 | Experimental

Fluid resuscitation influences cardiovascular performance and mortality in a murine model of sepsis

Authors: Sergio L. Zanotti-Cavazzoni, Massimiliano Guglielmi, Joseph E. Parrillo, Tracy Walker, R. Phillip Dellinger, Steven M. Hollenberg

Published in: Intensive Care Medicine | Issue 4/2009

Abstract

Rationale

Current murine models of sepsis do not account for the effects of aggressive fluid resuscitation on hemodynamics and mortality.

Objectives

Evaluate the impact of fluid resuscitation regimens on cardiovascular performance and survival in a murine model of sepsis.

Methods

Mice (n = 90) were made septic by cecal ligation and puncture (CLP), and received antibiotics plus Low, Intermediate, or High fluid resuscitation regimens. Stroke volume (SV), cardiac output (CO), and fractional shortening (FS) were measured by echocardiography at predefined time points.

Measurements and main results

Baseline echocardiographic measurements were similar in all groups. After CLP, SV and CO decreased early in all groups; High: 57.2 ± 9.2 to 23.9 ± 7.2 µL, and 26.8 ± 4.9 to 13.1 ± 5.8 ml/min; Intermediate: 52.1 ± 7.0 to 21.5 ± 6.6 µL, and 24.9 ± 4.1 to 11.9 ± 3.9 ml/min; Low: 54.0 ± 7.0 to 20.3 ± 5.6 µL, and 25.8 ± 4.0 to 11.3 ± 3.9 ml/min (P < 0.05 for all vs. baseline). With resuscitation there was a dose-dependent improvement in SV and CO (P < 0.05). At 24 h SV and CO were 44.0 ± 13.8 µL and 20.7 ± 8.5 ml/min in the High group, 39.8 ± 12.3 µL and 16.7 ± 6.5 ml/min in the Intermediate group, and 30.1 ± 12.4 µL and 14.0 ± 7.2 ml/min in the Low group. Survival was improved in the High fluid group (75%) compared to the Intermediate (58%) and the Low (35%) resuscitation groups (P < 0.05).

Conclusions

In this model, as in human sepsis, the intensity of fluid resuscitation modulates hemodynamic response and mortality. Incorporation of early and aggressive fluid resuscitation can significantly enhances the clinical relevance of murine models of sepsis.

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Title: Fluid resuscitation influences cardiovascular performance and mortality in a murine model of sepsis
Authors: Sergio L. Zanotti-Cavazzoni
Massimiliano Guglielmi
Joseph E. Parrillo
Tracy Walker
R. Phillip Dellinger
Steven M. Hollenberg
Publication date: 01-04-2009
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 4/2009
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-008-1360-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Fluid resuscitation influences cardiovascular performance and mortality in a murine model of sepsis

Abstract

Rationale

Objectives

Methods

Measurements and main results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Rationale

Objectives

Methods

Measurements and main results

Conclusions

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