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01-05-2008 | Experimental

Aging increases the susceptibility to injurious mechanical ventilation

Authors: Nicolás Nin, José A. Lorente, Marta De Paula, Pilar Fernández-Segoviano, Oscar Peñuelas, Alberto Sánchez-Ferrer, Leticia Martínez-Caro, Andrés Esteban

Published in: Intensive Care Medicine | Issue 5/2008

Abstract

Objective

To test the hypothesis that aging increases the susceptibility to organ dysfunction and systemic inflammation induced by injurious mechanical ventilation.

Design and setting

Experimental study in an animal model of ventilator-induced lung injury in the animal research laboratory in a university hospital.

Methods

Young (3–4 months old) and old (22–24 months old) anesthetized Wistar rats were ventilated for 60 min with a protective lung strategy (V_T = 9 ml/kg and PEEP = 5 cm H₂O, control) or with an injurious strategy (V_T = 35 ml/kg and PEEP = 0 cm H₂O, overventilated; n = 6 for each group).

Measurements and results

Mean arterial pressure and airway pressures (P_AW) were monitored. Arterial blood gases and serum AST, ALT, lactate, and IL-6 were measured. Vascular rings from the thoracic aorta were mounted in organ baths for isometric tension recording. We studied relaxations induced by acetylcholine (10 nM–10 μM) in norepinehrine-precontracted rings, and contractions induced by norepinephrine (1 nM–10 μM) in resting vessels. Lungs were examined by light microscopy. Injurious ventilation in young rats was associated with hypoxemia, lactic metabolic acidosis, increased serum AST, hypotension, impairment in norepinephrine and acetylcholine-induced vascular responses ex vivo and hyaline membrane formation. The high-V_T induced hypotension, increase in mean P_AW, AST, and IL-6, and the impairment in acetylcholine-induced responses were significantly more marked in aged than in young rats.

Conclusions

Elderly rats showed increased susceptibility to injurious mechanical ventilation-induced pulmonary injury, vascular dysfunction, and systemic inflammation.

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Title: Aging increases the susceptibility to injurious mechanical ventilation
Authors: Nicolás Nin
José A. Lorente
Marta De Paula
Pilar Fernández-Segoviano
Oscar Peñuelas
Alberto Sánchez-Ferrer
Leticia Martínez-Caro
Andrés Esteban
Publication date: 01-05-2008
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 5/2008
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-007-0960-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Aging increases the susceptibility to injurious mechanical ventilation

Abstract

Objective

Design and setting

Methods

Measurements and results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Design and setting

Methods

Measurements and results

Conclusions

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