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Intensive Care Medicine

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01-05-2003 | Experimental

Detrimental effects of short-term mechanical ventilation on diaphragm function and IGF-I mRNA in rats

Authors: Ghislaine Gayan-Ramirez, Kristel de Paepe, Pascal Cadot, Marc Decramer

Published in: Intensive Care Medicine | Issue 5/2003

Abstract

Objectives

Because respiratory muscle weakness appears to play an important role in weaning from mechanical ventilation, we developed an animal model of mechanical ventilation with appropriate controls in order to determine whether 24 h of mechanical ventilation already affected diaphragmatic function.

Design and interventions

Fifty-two male Wistar rats were randomized into three groups: a non-anesthetized control group (C, n=10), an anesthetized spontaneously breathing group (SB, n=9 out of 26), and an anesthetized and mechanically ventilated group (MV, n=12 out of 16).

Results

After 24 h, in vitro diaphragmatic force was decreased in SB group but even more so in MV group (i.e., 80 Hz: −15% in SB, P<0.005 vs C and −34% in MV group, P<0.005 vs C and SB). This was associated with a significant decrease in the diaphragm type I and type IIa dimensions in the SB group, which was more pronounced in the MV group. Interestingly, diaphragm IGF-I mRNA was decreased in the SB group (−14%, P<0.05 vs C), but more so in MV group (−29%, P<0.001 vs C and P<0.01 vs SB). Moreover, there was a significant correlation between diaphragm force and IGF-I mRNA (at 80 Hz r=0.51, P=0.0056).

Conclusions

We conclude that 24 h of mechanical ventilation in rats, independently of anesthesia, already significantly reduced diaphragm force, fiber dimensions, and its IGF-I mRNA levels.

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Title: Detrimental effects of short-term mechanical ventilation on diaphragm function and IGF-I mRNA in rats
Authors: Ghislaine Gayan-Ramirez
Kristel de Paepe
Pascal Cadot
Marc Decramer
Publication date: 01-05-2003
Publisher: Springer Berlin Heidelberg
Published in: Intensive Care Medicine / Issue 5/2003
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-003-1688-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Detrimental effects of short-term mechanical ventilation on diaphragm function and IGF-I mRNA in rats

Abstract

Objectives

Design and interventions

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Design and interventions

Results

Conclusions

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