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Intensive Care Medicine

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01-03-2003 | Experimental

Comparison of gravimetric and a double-indicator dilution technique for assessment of extra-vascular lung water in endotoxaemia

Authors: P. Rossi, A. Oldner, M. Wanecek, L. G. Leksell, A. Rudehill, D. Konrad, E. Weitzberg

Published in: Intensive Care Medicine | Issue 3/2003

Abstract

Objective.

To compare a molecular double-indicator dilution technique with the gravimetrical reference method for measurement of extra-vascular lung water in porcine endotoxin shock.

Design.

Open comparative experimental study.

Setting.

Animal research laboratory.

Measurements and results.

In fourteen anaesthetised, mechanically ventilated landrace pigs, central and pulmonary haemodynamics as well as pulmonary gas exchange were measured. Extra-vascular lung water was quantitated gravimetrically as well as with a molecular double indicator dilution technique. Eight of these animals were subjected to endotoxaemia, the rest serving as sham controls. No difference in extra-vascular lung water was observed between the two methods in sham animals. Furthermore, extra-vascular lung water assessed with the molecular double-indicator dilution technique at the initiation of endotoxin infusion did not differ significantly from the corresponding values for sham animals. Endotoxaemia induced a hypodynamic shock with concurrent pulmonary hypertension and a pronounced deterioration in gas exchange. No increase in extra-vascular lung water was detected with the molecular double-indicator dilution technique in response to endotoxin, whereas this parameter was significantly higher when assessed with the gravimetric method.

Conclusion.

The molecular double-indicator dilution technique showed similar results as the gravimetrical method for assessment of extra-vascular lung water in non-endotoxaemic conditions. However, during endotoxin-induced lung injury the molecular double indicator dilution technique failed to detect the significant increase in extra-vascular lung water as measured by the gravimetric method. These data suggest that the molecular double indicator dilution technique may be of limited value during sepsis-induced lung injury.

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Title: Comparison of gravimetric and a double-indicator dilution technique for assessment of extra-vascular lung water in endotoxaemia
Authors: P. Rossi
A. Oldner
M. Wanecek
L. G. Leksell
A. Rudehill
D. Konrad
E. Weitzberg
Publication date: 01-03-2003
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 3/2003
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-002-1604-z

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Comparison of gravimetric and a double-indicator dilution technique for assessment of extra-vascular lung water in endotoxaemia

Abstract

Objective.

Design.

Setting.

Measurements and results.

Conclusion.

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective.

Design.

Setting.

Measurements and results.

Conclusion.

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