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European Journal of Trauma and Emergency Surgery
Published in: European Journal of Trauma and Emergency Surgery 5/2023

09-06-2023 | Care | Original Article

Gut barrier protein levels in serial blood samples from critically ill trauma patients during and after intensive care unit stay

Authors: Hamiyet Donmez-Altuntas, Serap Sahin Ergul, Pinar Altin-Celik, Kadir Bulut, Hamiyet Eci̇roglu, Ramazan Uzen, Gulsah Gunes Sahin, Nurhayat Tugra Ozer, Sahin Temel, Turkmen Bahadir Arikan, Aliye Esmaoglu, Recep Civan Yuksel, Murat Sungur, Kursat Gundogan

Published in: European Journal of Trauma and Emergency Surgery | Issue 5/2023

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Abstract

Purpose

In an effort to better manage critically ill patients hospitalised in the intensive care unit (ICU) after experiencing multiple traumas, the present study aimed to assess whether plasma levels of intestinal epithelial cell barrier proteins, including occludin, claudin-1, junctional adhesion molecule (JAM-1), tricellulin and zonulin, could be used as novel biomarkers. Additional potential markers such as intestinal fatty acid-binding protein (I-FABP), d-lactate, lipopolysaccharide (LPS) and citrulline were also evaluated. We also aimed to determine the possible relationships between the clinical, laboratory, and nutritional status of patients and the measured marker levels.

Methods

Plasma samples from 29 patients (first, second, fifth and tenth days in the ICU and on days 7, 30 and 60 after hospital discharge) and 23 controls were subjected to commercial enzyme-linked immunosorbent assay (ELISA) testing.

Results

On first day (admission) and on the second day, plasma I-FABP, d-lactate, citrulline, occludin, claudin-1, tricellulin and zonulin levels were high in trauma patients and positively correlated with lactate, C-reactive protein (CRP), number of days of ICU hospitalisation, Acute Physiology and Chronic Health Evaluation II (APACHE II) score and daily Sequential Organ Failure Assessment (SOFA) scores (P < 0.05–P < 0.01).

Conclusion

The results of the present study showed that occludin, claudin-1, tricellulin and zonulin proteins, as well as I-FABP, d-lactate and citrulline, may be used as promising biomarkers for the evaluation of disease severity in critically ill trauma patients, despite the complexity of the analysis of various barrier markers. However, our results should be supported by future studies.
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Metadata
Title
Gut barrier protein levels in serial blood samples from critically ill trauma patients during and after intensive care unit stay
Authors
Hamiyet Donmez-Altuntas
Serap Sahin Ergul
Pinar Altin-Celik
Kadir Bulut
Hamiyet Eci̇roglu
Ramazan Uzen
Gulsah Gunes Sahin
Nurhayat Tugra Ozer
Sahin Temel
Turkmen Bahadir Arikan
Aliye Esmaoglu
Recep Civan Yuksel
Murat Sungur
Kursat Gundogan
Publication date
09-06-2023
Publisher
Springer Berlin Heidelberg
Keyword
Care
Published in
European Journal of Trauma and Emergency Surgery / Issue 5/2023
Print ISSN: 1863-9933
Electronic ISSN: 1863-9941
DOI
https://doi.org/10.1007/s00068-023-02298-6

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