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European Journal of Trauma and Emergency Surgery

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Open Access 01-04-2020 | Bone Defect | Original Article

From two stages to one: acceleration of the induced membrane (Masquelet) technique using human acellular dermis for the treatment of non-infectious large bone defects

Authors: René Danilo Verboket, Maximilian Leiblein, Maren Janko, Alexander Schaible, Jan Claas Brune, Katrin Schröder, Myriam Heilani, Charlotte Fremdling, Yannic Busche, Tanja Irrle, Ingo Marzi, Christoph Nau, Dirk Henrich

Published in: European Journal of Trauma and Emergency Surgery | Issue 2/2020

Abstract

Introduction

The induced membrane technique for the treatment of large bone defects is a two-step procedure. In the first operation, a foreign body membrane is induced around a spacer, then, in the second step, several weeks or months later, the spacer is removed and the Membrane pocket is filled with autologous bone material. Induction of a functional biological membrane might be avoided by initially using a biological membrane. In this study, the effect of a human acellular dermis (hADM, Epiflex, DIZG gGmbH) was evaluated for the treatment of a large (5 mm), plate-stabilised femoral bone defect.

Material and Methods

In an established rat model, hADM was compared to the two-stage induced membrane technique and a bone defect without membrane cover. Syngeneous spongiosa from donor animals was used for defect filling in all groups. The group size in each case was n = 5, the induction time of the membrane was 3–4 weeks and the healing time after filling of the defect was 8 weeks.

Results

The ultimate loads were increased to levels comparable with native bone in both membrane groups (hADM: 63.2% ± 29.6% of the reference bone, p < 0.05 vs. no membrane, induced membrane: 52.1% ± 25.8% of the reference bone, p < 0.05 vs. no membrane) and were significantly higher than the control group without membrane (21.5%). The membrane groups were radiologically and histologically almost completely bridged by new bone formation, in contrast to the control Group where no closed osseous bridging could be observed.

Conclusion

The use of the human acellular dermis leads to equivalent healing results in comparison to the two-stage induced membrane technique. This could lead to a shortened therapy duration of large bone defects.

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Title: From two stages to one: acceleration of the induced membrane (Masquelet) technique using human acellular dermis for the treatment of non-infectious large bone defects
Authors: René Danilo Verboket
Maximilian Leiblein
Maren Janko
Alexander Schaible
Jan Claas Brune
Katrin Schröder
Myriam Heilani
Charlotte Fremdling
Yannic Busche
Tanja Irrle
Ingo Marzi
Christoph Nau
Dirk Henrich
Publication date: 01-04-2020
Publisher: Springer Berlin Heidelberg
Keyword: Bone Defect
Published in: European Journal of Trauma and Emergency Surgery / Issue 2/2020
Print ISSN: 1863-9933
Electronic ISSN: 1863-9941
DOI: https://doi.org/10.1007/s00068-019-01296-x

2024 ASCO Annual Meeting

Springer Medicine

From two stages to one: acceleration of the induced membrane (Masquelet) technique using human acellular dermis for the treatment of non-infectious large bone defects

Abstract

Introduction

Material and Methods

Results

Conclusion

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Introduction

Material and Methods

Results

Conclusion

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