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01-04-2015 | Original Article

Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation

Authors: cand. med. Florian Simon, Jan-Oliver Dittmar, M.D., Stephan Brons, Ph. D., Lena Orschiedt, BTA, PD Steffi Urbschat, M.D., Prof. Klaus-Josef Weber, Ph.D., Prof. Jürgen Debus, M.D. Ph.D., Prof. Stephanie E. Combs, M.D., Stefan Rieken, M.D.

Published in: Strahlentherapie und Onkologie | Issue 4/2015

Abstract

Purpose

Sublethal doses of photon irradiation (IR) are suspected to increase tumor cell migration and support locoregional recurrence of disease, which has already been shown in other cell lines. This manuscript describes the effect of photon and carbon-ion IR on WHO class I meningioma cell migration and provides an approach to the underlying cellular mechanisms.

Materials and methods

Meningioma cells were gained operatively at the university hospital in Homburg/Saar, Germany. For migration, membranes (8-µm pore sizes) were coated with collagen I, with collagen IV, and with fibronectin. Cells were analyzed in migration experiments with or without serum stimulation, with or without photon and carbon IR 24 h prior to experiments, and with or without integrin antibodies. Fluorescence-activated cell sorting (FACS) analyses of the integrins ανβ₁, ανβ₃, and ανβ₅ were performed without IR and 6, 12 and 24 h after IR. Enzyme-linked immunosorbent assay (ELISA) analyses of matrix metalloproteinases (MMP)-2 and MMP-9 were realized with and without IR after cells were cultured on collagen I, collagen IV, or fibronectin for 24 h. Cells and supernatants for FACS and ELISA were stored at − 18 °C. The significance level was set at 5 % using both Student’s t test and two-way ANOVA.

Results

Migration of meningioma cells was serum-inducible (p < 0.001). It could be increased by photon IR (p < 0.02). The integrins ανβ₁ and ανβ₅ showed a 21 and 11 % higher expression after serum stimulation (not significant), respectively, and ανβ₁ expression was raised by 14 % (p = 0.0057) after photon IR. Antibody blockage of the integrins ανβ₁ and ανβ₅ inhibited serum- and photon-induced migration. Expression of MMP-2 and MMP-9 remained unchanged after both IR and fetal bovine serum (FBS). Carbon-ion IR left both integrin expression and meningioma cell migration unaffected.

Conclusion

Photon but not carbon-ion IR promotes serum-based meningioma cell migration. Fibronectin receptor integrin ανβ₁ signaling can be identified as an important mechanism for serum- and photon-induced migration of WHO class I meningioma cells.

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Title: Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation
Authors: cand. med. Florian Simon
Jan-Oliver Dittmar, M.D.
Stephan Brons, Ph. D.
Lena Orschiedt, BTA
PD Steffi Urbschat, M.D.
Prof. Klaus-Josef Weber, Ph.D.
Prof. Jürgen Debus, M.D. Ph.D.
Prof. Stephanie E. Combs, M.D.
Stefan Rieken, M.D.
Publication date: 01-04-2015
Publisher: Springer Berlin Heidelberg
Published in: Strahlentherapie und Onkologie / Issue 4/2015
Print ISSN: 0179-7158
Electronic ISSN: 1439-099X
DOI: https://doi.org/10.1007/s00066-014-0778-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Integrin-based meningioma cell migration is promoted by photon but not by carbon-ion irradiation

Abstract

Purpose

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusion

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