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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie 4/2023

Open Access 30-07-2021 | Original Article

Use of bioluminescence measurements for detection of artificial demineralization adjacent to orthodontic brackets

Authors: Prof. Anahita Jablonski-Momeni, Janine Sambale, Laura Gaerttner, Romy Nothelfer, Heike Korbmacher-Steiner

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Issue 4/2023

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Abstract

Purpose

Enamel demineralization can occur as a side effect during orthodontic treatment with fixed appliances and should be detected as early as possible. A new approach to assess demineralization is a system consisting of a photosensitive protein that binds to free calcium ions at the enamel surface. A camera is then used to visualize the bioluminescence spots. This in vitro study aimed to evaluate the ability of the bioluminescence technology to assess artificially demineralized enamel adjacent to various orthodontic brackets.

Methods

In all, 108 human enamel samples were allocated randomly to groups with different orthodontic bracket material: stainless steel, titanium, ceramic. Initial lesions were created adjacent to the brackets. The samples were assessed by bioluminescence before and after demineralization. Images were assessed for presence of bioluminescence spots (yes/no). To quantify the bioluminescence measurements, the images’ pixel values (P) were calculated within a defined area (F) adjacent to each bracket before and after demineralization. Quantitative light-induced fluorescence measurements (ΔF, ΔQ) were performed as the reference standard for demineralization.

Results

After demineralization, bioluminescence spots were visible (yes/no decision) in 87% of the samples. The pixel analysis of the bioluminescence spots showed significantly higher pixel values after demineralization compared to baseline (p < 0.0001). The bracket material had no influence on the bioluminescence measurements. All samples showed fluorescence loss with a median ΔF of −9.52% (±3.15) and a median ΔQ of −1.01% × mm2 (±3.34), respectively.

Conclusion

The bioluminescence technology is a promising tool to demonstrate demineralization adjacent to different orthodontic brackets in vitro.
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Metadata
Title
Use of bioluminescence measurements for detection of artificial demineralization adjacent to orthodontic brackets
Authors
Prof. Anahita Jablonski-Momeni
Janine Sambale
Laura Gaerttner
Romy Nothelfer
Heike Korbmacher-Steiner
Publication date
30-07-2021
Publisher
Springer Medizin
Published in
Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue 4/2023
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI
https://doi.org/10.1007/s00056-021-00341-y

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