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Inflammation Research
Published in: Inflammation Research 10/2015

01-10-2015 | Original Research Paper

NZ suppresses TLR4/NF-κB signalings and NLRP3 inflammasome activation in LPS-induced RAW264.7 macrophages

Authors: Pengjun Xiang, Tong Chen, Yi Mou, Hui Wu, Peng Xie, Guo Lu, Xiaojian Gong, Qinghua Hu, Yihua Zhang, Hui Ji

Published in: Inflammation Research | Issue 10/2015

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Abstract

Objective

The purpose of the present study was to evaluate the potential therapeutic effects of NZ on lipopolysaccharide (LPS)-induced RAW264.7 cells and explore its underlying mechanisms.

Methods

The effect of NZ on NO generation in LPS-activated macrophage was measured by Griess assay. The concentrations of TNF-α, IL-18, IL-1β were analyzed with ELISA kits. The LPS-induced production of reactive oxygen species (ROS) was determined by flow cytometry. The protein expressions of TLR4, NF-κB and NLRP3 signaling pathway were investigated with Western blot analysis.

Results

It was shown that NZ significantly reduced the production of NO and the generation of pro-inflammatory cytokines in LPS-induced RAW264.7 cells. In addition, NZ markedly inhibited the up-regulation of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and the activation of nuclear factor kappa B (NF-κB) in LPS-stimulated RAW 264.7 macrophages. Of note, NZ suppressed the expression of the inflammasome component such as NOD-like receptor 3(NLRP3), apoptosis-associated speck-like protein containing CARD(ASC), as well as the levels of cytokines including Interleukin-18(IL-18) and Interleukin-1β(IL-1β).

Conclusion

These results indicated that NZ inhibited the generations of NO and pro-inflammatory cytokines by suppressing TLR4/MyD88/NF-κB pathway, suggesting that NZ could be an effective candidate for ameliorating LPS-induced inflammatory responses.
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Metadata
Title
NZ suppresses TLR4/NF-κB signalings and NLRP3 inflammasome activation in LPS-induced RAW264.7 macrophages
Authors
Pengjun Xiang
Tong Chen
Yi Mou
Hui Wu
Peng Xie
Guo Lu
Xiaojian Gong
Qinghua Hu
Yihua Zhang
Hui Ji
Publication date
01-10-2015
Publisher
Springer Basel
Published in
Inflammation Research / Issue 10/2015
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-015-0863-4

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