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Inflammation Research
Published in: Inflammation Research 2/2012

01-02-2012 | Short Communication

Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products

Authors: Young Hee Nam, Arim Min, Seong Hoon Kim, Young Ah Lee, Kyeong Ah Kim, Kyoung-Ju Song, Myeong Heon Shin

Published in: Inflammation Research | Issue 2/2012

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Abstract

Objective and method

Trichomonas vaginalis is a flagellated protozoan parasite that causes human trichomoniasis. Although T. vaginalis itself can secrete lipid mediator leukotriene (LT) B4 leading to neutrophil activation, information regarding the signaling mechanism involved in neutrophil activation induced by T. vaginalis-secreted LTB4 is limited. We investigated whether LTB4 contained in the T. vaginalis-derived secretory products (TvSP) is closely involved in interleukin (IL)-8 production in human neutrophils via LTB4 receptors BLT1 or BLT2.

Results

T. vaginalis produced more than 714 pg/ml of LTB4 per 1 × 107 trichomonads. The ability of trichomonads to secrete LTB4 was inhibited by treatment of trichomonads with the 5-lipo-oxygenease inhibitor AA861, but not the cyclo-oxygenease I inhibitor FR122047. When neutrophils were incubated with TvSP obtained from 1 × 107 trichomonads, IL-8 protein secretion was significantly increased compared to results for cells incubated with medium alone. The stimulatory effect of TvSP on IL-8 production was strongly inhibited by pretreatment of TvSP with lipase, although pretreatment with heat or proteinase K showed little inhibitory effect. Moreover, TvSP-induced IL-8 production was efficiently inhibited when trichomonads were pretreated with AA861 or when neutrophils were pretreated with antagonists for BLT1 or BLT2.

Conclusion

Our results suggest that LTB4 receptors BLT1 and BLT2 are involved in IL-8 production in neutrophils induced by T. vaginalis.
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Metadata
Title
Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products
Authors
Young Hee Nam
Arim Min
Seong Hoon Kim
Young Ah Lee
Kyeong Ah Kim
Kyoung-Ju Song
Myeong Heon Shin
Publication date
01-02-2012
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 2/2012
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-011-0425-3

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