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Inflammation Research
Published in: Inflammation Research 7/2010

01-07-2010 | Original Research Paper

Hyaluronic acid inhibits nitric oxide-induced apoptosis and dedifferentiation of articular chondrocytes in vitro

Authors: Hao Peng, Jian-lin Zhou, Shi-qing Liu, Qiong-jie Hu, Jiang-hua Ming, Bo Qiu

Published in: Inflammation Research | Issue 7/2010

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Abstract

Objective

Nitric oxide is an important mediator in Osteoarthritis (OA), and causes apoptosis and dedifferentiation in articular chondrocytes. Protein kinase Cα is involved in modulating apoptosis and dedifferentiation of articular chondrocytes induced by nitric oxide. Hyaluronic acid is widely used in the treatment of osteoarthritis and exerts significant chondroprotective effects. The exact mechanisms of its chondroprotective action are not yet fully elucidated. The present study was performed to investigate the effects and mechanisms of hyaluronic acid in NO-induced apoptosis and dedifferentiation of chondrocytes.

Methods

The ratio of apoptotic cell and cell viability was surveyed by TUNEL, MTT assay and flow cytometry. The expression of aggrecan, type II collagen, and PKCα were determined by real-time PCR and Western blot. The expression changes of caspase-3 and bcl-2 was detected by Western blot. The mitochondrial membrane potential (ΔΨm) was evaluated by Rhodamine-123 fluorescence.

Results

HA reduces the TUNEL positive cell, nuclei fragment and the impairment of ΔΨm. NO-induced chondrocyte dedifferentiation was blocked by HA, which restores expression of aggrecan and type II collagen of chondrocytes and cell viability. HA can block inhibition of PKC-α by NO.

Conclusion

Our results show that HA blocks NO-induced apoptosis and dedifferentiation of articular chondrocytes by modulation of ΔΨm and PKCα.
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Metadata
Title
Hyaluronic acid inhibits nitric oxide-induced apoptosis and dedifferentiation of articular chondrocytes in vitro
Authors
Hao Peng
Jian-lin Zhou
Shi-qing Liu
Qiong-jie Hu
Jiang-hua Ming
Bo Qiu
Publication date
01-07-2010
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 7/2010
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-010-0156-x

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