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02-04-2024 | Echinococcosis | Original Article

Molecular characterization of cystic echinococcosis cysts isolated from cattle in Shiraz, Iran

Authors: Mohammad Zare, Seyed Mahmoud Sadjjadi, Fattaneh Mikaeili, Manouchehr Shirani, Yosef Sharifi, Aref Teimouri

Published in: Journal of Parasitic Diseases

Abstract

Cystic echinococcosis (CE), is one of the common parasitic infections of domestic animals and humans caused by the larval stages of the tapeworm Echinococcus granulosus. This research aimed to identify of CE cysts in cattle isolates from Shiraz slaughterhouse using molecular technique. Thirty CE cysts isolates were collected and total DNA was extracted from protoscolices or the germinal layer of each cyst followed by polymerase chain reaction (PCR) using cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit 1 (NADH-1) mitochondrial genes (~ 450 bp) and sequencing. The sequence results were compared with available reference sequences in GenBank by BLAST system. Phylogenetic analysis was performed using MEGA 5.0 software and the maximum likelihood method. The results showed that all 30 samples examined in this study were identified as E. granulosus sensu stricto (G1–G3 genotypes) with G1 dominant.

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Title: Molecular characterization of cystic echinococcosis cysts isolated from cattle in Shiraz, Iran
Authors: Mohammad Zare
Seyed Mahmoud Sadjjadi
Fattaneh Mikaeili
Manouchehr Shirani
Yosef Sharifi
Aref Teimouri
Publication date: 02-04-2024
Publisher: Springer India
Keywords: Echinococcosis
Polymerase Chain Reaction
Published in: Journal of Parasitic Diseases
Print ISSN: 0971-7196
Electronic ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-024-01655-5

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