Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Head and Neck Pathology
Published in: Head and Neck Pathology 1/2024

01-12-2024 | Leukoplakia | Research

Expression Profile of Circulating Exosomal microRNAs in Leukoplakia, Oral Submucous Fibrosis, and Combined Lesions of Leukoplakia and Oral Submucous Fibrosis

Authors: Dinesh Yasothkumar, Pratibha Ramani, Selvaraj Jayaraman, Karthikeyan Ramalingam, W. M. Tilakaratne

Published in: Head and Neck Pathology | Issue 1/2024

Login to get access

Abstract

Objectives

This study aims to elucidate the expression of circulating exosomal miRNAs miRNA 21, miRNA 184, and miRNA 145 in the studied groups, including patients with (i) leukoplakia; (ii) oral submucous fibrosis; (iii) oral submucous fibrosis with leukoplakia; (iv) oral squamous cell carcinoma; and (v) healthy individuals.

Study Design

An observational study was conducted among 54 patients who reported to the outpatient department of Saveetha Dental College and Hospitals. The patients were divided into three groups: Group I healthy individuals (n = 18), Group II: case group (leukoplakia, OSMF, and leukoplakia and OSMF) (n = 18), and Group III: OSCC (n = 18). Real-time polymerase chain reaction analysis was carried out to assess the expression profiles of miRNA 21, miRNA 184, and miRNA 145. The statistical analysis was calculated using SPSS software version 23.

Results

All three miRNAs showed a statistically significant difference in the one-way ANOVA test between the case group (leukoplakia, OSMF, and leukoplakia and OSMF), healthy group, and OSCC group (p < 0.005). The case group (leukoplakia, OSMF, leukoplakia and OSMF) showed upregulated expression of miRNA 21 and miRNA 184 with threefold change and fourfold change and downregulated expression of miRNA 145 with 1.5-fold change when compared to apparently healthy individuals.

Conclusion

Plasma circulating exosomal miRNAs miRNA 21, miRNA 145, and miRNA 184 expression could be a novel panel of plasma biomarkers to categorise case group (leukoplakia, OSMF, leukoplakia and OSMF) patients with a high risk of malignant transformation.
Appendix
Available only for authorised users
Literature
1.
2.
Iocca O et al (2020) Potentially malignant disorders of the oral cavity and oral dysplasia: a systematic review and meta-analysis of malignant transformation rate by subtype. Head Neck 42:539–555PubMedCrossRef
3.
Sabarinath B, Sriram G, Saraswathi TR, Sivapathasundharam B (2011) Immunohistochemical evaluation of mast cells and vascular endothelial proliferation in oral submucous fibrosis. Indian J Dent Res 22:116–121PubMedCrossRef
4.
5.
Pitiyage G, Tilakaratne WM, Tavassoli M, Warnakulasuriya S (2009) Molecular markers in oral epithelial dysplasia: review. J Oral Pathol Med 38:737–752PubMedCrossRef
6.
Lauritano D et al (2019) Liquid biopsy in head and neck squamous cell carcinoma: prognostic significance of circulating tumor cells and circulating tumor DNA. A systematic review. Oral Oncol 97:7–17PubMedCrossRef
7.
Yang W-Y et al (2020) Liquid biopsy in head and neck squamous cell carcinoma: circulating tumor cells, circulating tumor DNA, and exosomes. Expert Rev Mol Diagn 20:1213–1227PubMedCrossRef
8.
Yap T et al (2020) Extracellular vesicles in oral squamous cell carcinoma and oral potentially malignant disorders: a systematic review. Int J Mol Sci 21:1197PubMedPubMedCentralCrossRef
9.
Munson PB, Shukla A (2018) Introduction to exosomes and cancer. Diagnostic and Therapeutic Applications of Exosomes in Cancer. Academic Press, London, pp 1–10
10.
Lima LG, Möller A (2018) Biodistribution of cancer-derived exosomes. Diagnostic and Therapeutic Applications of Exosomes in Cancer. Academic Press, London, pp 175–186CrossRef
11.
Hochendoner P, Zhao Z, He M (2018) Diagnostic potential of tumor exosomes. Diagnostic and Therapeutic Applications of Exosomes in Cancer. Academic Press, London, pp 161–1735CrossRef
12.
13.
Sakha S, Muramatsu T, Ueda K, Inazawa J (2016) Exosomal microRNA miR-1246 induces cell motility and invasion through the regulation of DENND2D in oral squamous cell carcinoma. Sci Rep 6:38750PubMedPubMedCentralCrossRef
14.
15.
16.
17.
Sempere LF (2019) MicroRNA as biomarkers in cancer diagnostics and therapy
18.
Maheswari TNU, Venugopal A, Sureshbabu NM, Ramani P (2018) Salivary micro RNA as a potential biomarker in oral potentially malignant disorders: a systematic review. Tzu Chi Med J 30:55–60PubMedCentralCrossRef
19.
Ribas J et al (2012) A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts. Nucleic Acids Res 40:6821–6833PubMedPubMedCentralCrossRef
20.
21.
Chan JA, Krichevsky AM, Kosik KS (2005) MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res 65:6029–6033PubMedCrossRef
22.
Wong T-S et al (2008) Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clin Cancer Res 14:2588–2592PubMedCrossRef
23.
Wong T-S, Ho W-K, Chan JY-W, Ng RW-M, Wei WI (2009) Mature miR-184 and squamous cell carcinoma of the tongue. SciWorldJ 9:130–132
24.
Chen D et al (2018) miR-184 promotes cell proliferation in tongue squamous cell carcinoma by targeting SOX7. Oncol Lett 16:2221–2228PubMedPubMedCentral
25.
26.
Michael MZ, O’Connor SM, van Holst Pellekaan NG, Young GP, James RJ (2003) Reduced accumulation of specific microRNAs in colorectal neoplasia. Mol Cancer Res 1:882–891PubMed
27.
Pagliuca A et al (2013) Analysis of the combined action of miR-143 and miR-145 on oncogenic pathways in colorectal cancer cells reveals a coordinate program of gene repression. Oncogene 32:4806–4813PubMedCrossRef
28.
Warnakulasuriya S et al (2020) Oral potentially malignant disorders: a consensus report from an international seminar on nomenclature and classification, convened by the WHO Collaborating Centre for Oral Cancer. Oral Dis 27:1862–1880PubMedCrossRef
29.
30.
Mello FW et al (2018) Prevalence of oral potentially malignant disorders: a systematic review and meta-analysis. J Oral Pathol Med 47:633–640PubMedCrossRef
31.
32.
Murthy V et al (2022) Malignant transformation rate of oral submucous fibrosis: a systematic review and meta-analysis. J Clin Med Res 11:1793
33.
Hande A et al (2018) Role of hypoxia in malignant transformation of oral submucous fibrosis. J Datta Meghe Inst Med Sci Univ 13:38CrossRef
34.
Ho MWS (2013) Patients with both oral leukoplakia and oral submucous fibrosis had a higher rate of malignant transformation compared to patients with oral leukoplakia or oral submucous fibrosis alone. J Evid Based Dent Pract 13:183–184PubMedCrossRef
35.
Jayasooriya PR, Nadeeka Jayasinghe KA, Mudiyanselage Tilakaratne W (2011) Relationship between thickness of fibrosis and epithelial dysplasia in oral submucous fibrosis. J Investig Clin Dent 2:171–175PubMedCrossRef
36.
Raisch J, Darfeuille-Michaud A, Nguyen HTT (2013) Role of microRNAs in the immune system, inflammation and cancer. World J Gastroenterol 19:2985–2996PubMedPubMedCentralCrossRef
37.
Zahran F, Ghalwash D, Shaker O, Al-Johani K, Scully C (2015) Salivary microRNAs in oral cancer. Oral Dis 21:739–747PubMedCrossRef
38.
Dioguardi M et al (2022) MicroRNA-21 expression as a prognostic biomarker in oral cancer: systematic review and meta-analysis. Int J Environ Res Public Health 19:3396PubMedPubMedCentralCrossRef
39.
Han M et al (2012) Antagonism of miR-21 reverses epithelial-mesenchymal transition and cancer stem cell phenotype through AKT/ERK1/2 inactivation by targeting PTEN. PLoS ONE 7:e39520PubMedPubMedCentralCrossRef
40.
Liu Z-L, Wang H, Liu J, Wang Z-X (2013) MicroRNA-21 (miR-21) expression promotes growth, metastasis, and chemo- or radioresistance in non-small cell lung cancer cells by targeting PTEN. Mol Cell Biochem 372:35–45PubMedCrossRef
41.
Asangani IA et al (2008) MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene 27:2128–2136PubMedCrossRef
42.
43.
Zhang J et al (2013) Loss of microRNA-143/145 disturbs cellular growth and apoptosis of human epithelial cancers by impairing the MDM2-p53 feedback loop. Oncogene 32:61–69PubMedCrossRef
44.
45.
Kawakita A et al (2014) MicroRNA-21 promotes oral cancer invasion via the Wnt/β-catenin pathway by targeting DKK2. Pathol Oncol Res 20:253–261PubMedCrossRef
46.
Han L et al (2012) MicroRNA-21 expression is regulated by β-catenin/STAT3 pathway and promotes glioma cell invasion by direct targeting RECK. CNS Neurosci Ther 18:573–583PubMedPubMedCentralCrossRef
47.
González-Moles MA, Ruiz-Ávila I, Gil-Montoya JA, Plaza-Campillo J, Scully C (2014) β-catenin in oral cancer: an update on current knowledge. Oral Oncol 50:818–824PubMedCrossRef
48.
Xin Z, Tong Z, Tan J, Liu C (2021) MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma. Exp Ther Med 21:373PubMedPubMedCentralCrossRef
49.
Uma Maheswari TN, Nivedhitha MS, Ramani P (2020) Expression profile of salivary micro RNA-21 and 31 in oral potentially malignant disorders. Braz Oral Res 34:e002PubMedCrossRef
50.
Prasad SR, Pai A, Shyamala K, Yaji A (2020) Expression of salivary miRNA 21 in oral submucous fibrosis (OSMF): an observational study. Microrna 9:295–302PubMedPubMedCentralCrossRef
51.
Kaunein N et al (2021) A systematic review of MicroRNA signatures associated with the progression of leukoplakia with and without epithelial dysplasia. Biomolecules 11:1879PubMedPubMedCentralCrossRef
52.
Cervigne NK et al (2009) Identification of a microRNA signature associated with progression of leukoplakia to oral carcinoma. Hum Mol Genet 18:4818–4829PubMedCrossRef
53.
Chen C-M et al (2021) Exosome-derived microRNAs in oral squamous cell carcinomas impact disease prognosis. Oral Oncol 120:105402PubMedCrossRef
54.
Yoon AJ et al (2020) MicroRNA-based risk scoring system to identify early-stage oral squamous cell carcinoma patients at high-risk for cancer-specific mortality. Head Neck 42:1699–1712PubMedPubMedCentralCrossRef
55.
Mahmood N et al (2019) Circulating miR-21 as a prognostic and predictive biomarker in oral squamous cell carcinoma. Pak J Med Sci Q 35:1408–1412
Metadata
Title
Expression Profile of Circulating Exosomal microRNAs in Leukoplakia, Oral Submucous Fibrosis, and Combined Lesions of Leukoplakia and Oral Submucous Fibrosis
Authors
Dinesh Yasothkumar
Pratibha Ramani
Selvaraj Jayaraman
Karthikeyan Ramalingam
W. M. Tilakaratne
Publication date
01-12-2024
Publisher
Springer US
Published in
Head and Neck Pathology / Issue 1/2024
Electronic ISSN: 1936-0568
DOI
https://doi.org/10.1007/s12105-024-01627-4

Other articles of this Issue 1/2024

Head and Neck Pathology 1/2024 Go to the issue

Research

Immunophenotypic and Gene Expression Analyses of the Inflammatory Microenvironment in High-Grade Oral Epithelial Dysplasia and Oral Lichen Planus

Research

Chronic Sclerosing Sialadenitis of the Submandibular Gland and its Histopathological Spectrum in the IgG4-Related Disease: a Series of 17 Cases

Review

Dynamic Role of miRNAs in Salivary Gland Carcinomas: From Biomarkers to Therapeutic Targets

Case Report

Primary Oral Mixed Neuroendocrine–Non-neuroendocrine Neoplasm (MiNEN): A Rare Case Report and Review of the Literature

Review

A Comparison of Clear Cell Sarcoma to Jaw and Salivary Tumors Bearing EWS Fusions

Image

Traumatic Ulcerative Granuloma with Stromal Eosinophilia on the Tongue