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Surgery Today
Published in: Surgery Today 12/2017

01-12-2017 | Original Article

LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review

Authors: Kensuke Yamamura, Keisuke Kosumi, Yoshifumi Baba, Kazuto Harada, Feng Gao, Xiaobo Zhang, Lei Zhou, Yuki Kitano, Kota Arima, Takayoshi Kaida, Hideaki Takeyama, Takaaki Higashi, Katsunori Imai, Daisuke Hashimoto, Akira Chikamoto, Xiaodong Tan, Hideo Baba

Published in: Surgery Today | Issue 12/2017

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Abstract

Purpose

Global DNA hypomethylation plays an important role in genomic instability and carcinogenesis. The long interspersed nucleotide element-1 (LINE-1) methylation level is a good surrogate marker of the global DNA methylation level. Previously, we demonstrated a strong relationship between LINE-1 hypomethylation and poor prognosis in certain cancers. However, the relationship between the LINE-1 methylation level and the clinical outcome of pancreatic cancer (PC) remains unclear.

Methods

We used a pyrosequencing assay to measure LINE-1 methylation levels in 126 samples of resected PC and evaluated the prognostic value of the LINE-1 methylation level.

Results

LINE-1 methylation levels were significantly lower in PC tissues than in matched noncancerous pancreatic tissues (p = 0.039, n = 36). The tumoral LINE-1 methylation range was 41.3–92.8 (n = 126, mean 77.7, median 78.5, standard deviation 5.7). The LINE-1 methylation level was unrelated to clinical and pathological features. Moreover, LINE-1 hypomethylation was not significantly associated with overall survival, cancer specific survival, or disease-free survival (log-rank p = 0.30, p = 0.18 and p = 0.50, respectively).

Conclusion

The LINE-1 methylation level appears not to be associated with poor prognosis in PC. The effect of the LINE-1 methylation level on the survival of PC patients needs to be confirmed in a larger-cohort study.
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Metadata
Title
LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review
Authors
Kensuke Yamamura
Keisuke Kosumi
Yoshifumi Baba
Kazuto Harada
Feng Gao
Xiaobo Zhang
Lei Zhou
Yuki Kitano
Kota Arima
Takayoshi Kaida
Hideaki Takeyama
Takaaki Higashi
Katsunori Imai
Daisuke Hashimoto
Akira Chikamoto
Xiaodong Tan
Hideo Baba
Publication date
01-12-2017
Publisher
Springer Japan
Published in
Surgery Today / Issue 12/2017
Print ISSN: 0941-1291
Electronic ISSN: 1436-2813
DOI
https://doi.org/10.1007/s00595-017-1539-1

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