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Neurological Sciences
Published in: Neurological Sciences 12/2014

01-12-2014 | Original Article

Lentivirus-mediated delivery of sonic hedgehog into the striatum stimulates neuroregeneration in a rat model of Parkinson disease

Authors: Yi Zhang, Weiren Dong, Suiqun Guo, Shu Zhao, Suifen He, Lihua Zhang, Yinjuan Tang, Haihong Wang

Published in: Neurological Sciences | Issue 12/2014

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Abstract

Parkinson disease (PD) is a progressive neurodegenerative disorder in which the nigrostriatal pathway, consisting of dopaminergic neuronal projections from the substantia nigra to the striatum, degenerates. Viral transduction is currently the most promising in vivo strategy for delivery of therapeutic proteins into the brain for treatment of PD. Sonic hedgehog (Shh) is necessary for cell proliferation, differentiation and neuroprotection in the central nervous system. In this study, we investigated the effects of overexpressed N-terminal product of SHH (SHH-N) in a PD model rat. A lentiviral vector containing SHH-N was stereotactically injected into the striatum 24 h after a striatal 6-OHDA lesion. We found that overexpressed SHH-N attenuated behavioral deficits and reduced the loss of dopamine neurons in the substantia nigra and the loss of dopamine fibers in the striatum. In addition, fluoro-ruby-labeled nigrostriatal projections were also repaired. Together, our results demonstrate the feasibility and efficacy of using the strategy of lentivirus-mediated Shh-N delivery to delay nigrostriatal pathway degeneration. This strategy holds the potential for therapeutic application in the treatment of PD.
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Metadata
Title
Lentivirus-mediated delivery of sonic hedgehog into the striatum stimulates neuroregeneration in a rat model of Parkinson disease
Authors
Yi Zhang
Weiren Dong
Suiqun Guo
Shu Zhao
Suifen He
Lihua Zhang
Yinjuan Tang
Haihong Wang
Publication date
01-12-2014
Publisher
Springer Milan
Published in
Neurological Sciences / Issue 12/2014
Print ISSN: 1590-1874
Electronic ISSN: 1590-3478
DOI
https://doi.org/10.1007/s10072-014-1866-6

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