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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-06-2010 | Basic Science

Lens epithelial cell apoptosis initiates diabetic cataractogenesis in the Zucker diabetic fatty rat

Authors: Junghyun Kim, Chan-Sik Kim, Eunjin Sohn, Hyojun Kim, Il-Ha Jeong, Jin Sook Kim

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 6/2010

Abstract

Background

It has been suggested that damage of lens epithelial cell (LEC) may play an important role in cataract formation. Nitric oxide is involved in cataract development. Here, we investigated the relationship between LEC damage and iNOS expression in the Zucker diabetic fatty (ZDF) rat.

Methods

At 21 weeks of age, the eyes were enucleated and the lens opacity was then examined. Apoptosis were detected by TUNEL assay, and the expression of iNOS and NF-κB activation were studied by immunohistochemistry and southwestern histochemistry respectively.

Results

In 21-week-old male ZDF rats, cataract was developed, TUNEL-positive LECs were markedly increased, and the expression levels of iNOS mRNA and protein were significantly upregulated. The expression pattern of iNOS was closely correlated with apoptotic change of LECs. In addition, advanced glycation end products (AGEs) were accumulated in cytoplasm of LECs. Activated NF-κB was mainly detected in nucleus of LECs.

Conclusions

The higher expressions of AGEs, NF-κB and iNOS in LECs of diabetic rats suggest that these factors are involved in apoptosis of LEC alterations related to diabetic cataract.

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Title: Lens epithelial cell apoptosis initiates diabetic cataractogenesis in the Zucker diabetic fatty rat
Authors: Junghyun Kim
Chan-Sik Kim
Eunjin Sohn
Hyojun Kim
Il-Ha Jeong
Jin Sook Kim
Publication date: 01-06-2010
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 6/2010
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-010-1313-1

At a glance: The STEP trials

Springer Medicine

Lens epithelial cell apoptosis initiates diabetic cataractogenesis in the Zucker diabetic fatty rat

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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