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Digestive Diseases and Sciences
Published in: Digestive Diseases and Sciences 12/2011

01-12-2011 | Original Article

Lemon Verbena Infusion Consumption Attenuates Oxidative Stress in Dextran Sulfate Sodium-Induced Colitis in the Rat

Authors: Loïc Lenoir, Adrien Rossary, Juliette Joubert-Zakeyh, Juliette Vergnaud-Gauduchon, Marie-Chantal Farges, Didier Fraisse, Odile Texier, Jean-Louis Lamaison, Marie-Paule Vasson, Catherine Felgines

Published in: Digestive Diseases and Sciences | Issue 12/2011

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Abstract

Background

Inflammatory bowel diseases (IBD) consist of an uncontrolled intestinal inflammation leading to mucosal disruption. This inflammation is accompanied by an excessive production of reactive oxygen species (ROS). Polyphenols are micronutrients with antioxidative and anti-inflammatory properties, and may play an interesting role in the prevention of intestinal inflammation. Lemon verbena (Aloysia triphylla) infusion is a popular herbal infusion rich in polyphenols (flavones and verbascoside).

Aims

This study evaluated the preventive effects of lemon verbena infusion consumption against mild-to-moderate dextran sulfate sodium (DSS)-induced colitis in rats.

Methods

Wistar rats drank water or lemon verbena infusion for 14 days. On day 15, half of the rats received DSS (4%) in their drink for 7 days. At the end of the experimental period, the colon was taken for histopathological examination and determination of myeloperoxidase (MPO) activity, antioxidant enzyme activities (superoxide dismutase [SOD], glutathione peroxidase [GPx], glutathione reductase [GR], catalase [CAT]), glutathione and lipid peroxidation. Lymphocyte populations were determined in blood, mesenteric nodes and Peyer’s patches.

Results

Rats ingested daily 5.6 μmol of polyphenols. DSS reduced food intake and induced colitis, as reflected by histological lesions and increased MPO activity. Although these alterations were not significantly counteracted by lemon verbena consumption, the herbal infusion increased colonic SOD activity and decreased lipid peroxidation (malondialdehyde). Other oxidative stress markers (GPx, GR, CAT, glutathione) were not significantly modified.

Conclusion

Our study shows that the preventive consumption of lemon verbena infusion offered some antioxidative protection during experimental colitis by stimulating SOD activity and decreasing lipid peroxidation.
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Metadata
Title
Lemon Verbena Infusion Consumption Attenuates Oxidative Stress in Dextran Sulfate Sodium-Induced Colitis in the Rat
Authors
Loïc Lenoir
Adrien Rossary
Juliette Joubert-Zakeyh
Juliette Vergnaud-Gauduchon
Marie-Chantal Farges
Didier Fraisse
Odile Texier
Jean-Louis Lamaison
Marie-Paule Vasson
Catherine Felgines
Publication date
01-12-2011
Publisher
Springer US
Published in
Digestive Diseases and Sciences / Issue 12/2011
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-011-1784-x

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