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01-04-2022 | Laser | Original Article

Photobiomodulation effect in tumoral necrosis factor-alpha(TNF-α) on the viability of random skin flap in rats

Authors: Georgia Rodrigues Esteves, Ivaldo Esteves Junior, Igor Fagioli Bordello Masson, Aline Fernanda Perez Machado, Maria Carolina Derencio Oliveira, Cristiano Schiavinato Baldan, Thiago Saikali Farcic, Richard Eloin Liebano, Hélio Plapler

Published in: Lasers in Medical Science | Issue 3/2022

Abstract

The aim of this study was to investigate the effect of red laser (660 nm) photobiomodulation (PBM) with different energies on tumor necrosis factor-alpha(TNF-α) expression for random skin flap viability in rats. Twenty-four Wistar rats were divided into three groups: sham group (SG), PBM laser group with an energy dose of 0.29 J (0.29G), and PBM laser group with an energy dose of 7.30 J (7.30G). A cranially based dorsal skin flap measuring 10 × 4 cm was raised and a plastic barrier was placed between the flap and its bed. PBM was applied in 3 timepoints: in the immediate postoperative period, in the 1^st and in the 2^nd postoperative days; the animals were euthanized on the 7^th postoperative day. The assessments included: TNF-α expression of 3 different flap areas (proximal, medial and distal), by immunohistochemistry; percentage of skin flap necrosis area, by the paper template method. The statistical analysis was performed through the Kruskal-Wallis and Mann-Whitney tests, the level of significance adopted was 5% (p < 0.05). TNF-α expression was significantly lower for 7.30G in the proximal area, reduced for SG in the medial point, and larger for 7.30G in the distal area. The percentage of flap necrosis area was significantly reduced for 7.30G. Higher energy doses are more efficacious than lower energy doses for modulating TNF-α expression. PBM with an energy dose of 7.30 J was effective in reducing the expression of TNF-α and increase skin flap viability.

Ferreira LM (1995) Retalhos cutâneos. In: Ferreira LM (ed) Manual de cirurgia plástica. Atheneu, São Paulo, pp 45–62

Kerrigan CL (1983) Skin flap failure: pathophysiology. Plast Reconstr Surg 72(6):766–777PubMedCrossRef

Liebano RE, Ferreira LM, Neto MS (2002) The effect of trans-cutaneous electrical nerve stimulation on the viability of random skin flaps in rats. Can J Plast Surg 10(4):151–154CrossRef

Esteves Junior I, Masson IB, Ferreira LM, Liebano RE, Baldan C, Gomes AC (2005) Administração tópica de cloridrato de hidralazina na viabilidade de retalho cutâneo randômico em ratos. Acta Cir Bras 20(2):164–167CrossRef

Prado RP, Liebano RE, Hochman B, Pinfildi CE, Ferreira LM (2006) Experimental model for low level laser therapy on ischemic random skin flap in rats. Acta Cir Bras 21(4):258–262PubMedCrossRef

Shalom A, Friedman T, Westreich M (2008) Effect of aspirin and heparin on random skin flap survival in rats. Dermatol Surg 34(6):785–790 discussion 790PubMed

Esteves Junior I, Tacani PM, Liggieri VC, Ruggi BG, Ferreira LM, Liebano RE (2009) Histamine iontophoresis on the viability of random skin flap in rats. Acta Cir Bras 24(1):48–51PubMedCrossRef

Pazoki-Toroudi H, Ajami M, Habibey R, Hajiaboli E, Firooz A (2009) The effect of enalapril on skin flap viability is independent of angiotensin II AT1 receptors. Ann Plast Surg 62(6):699–702PubMedCrossRef

Prado RP, Pinfildi CE, Liebano RE et al (2009) Effect of application site of low-level laser therapy in random cutaneous flap viability in rats. Photomed Laser Surg 27:411–416PubMedCrossRef

10.

Baldan CS, Marques AP, Schiavinato AM, Casarotto RA (2012) The effects of different doses of 670 nm diode laser on skin flap survival in rats. Acta Cir Bras 27(2):155–161PubMedCrossRef

11.

Baldan CS, Masson IF, Esteves Júnior I, Baldan AM, Machado AF, Casaroto RA, Liebano RE (2015) Inhibitory effects of low-level laser therapy on skin-flap survival in a rat model. Plast Surg (Oakv) 23(1):35–39CrossRef

12.

Kubota J, Oshiro T (1996) Effects of diode Laser therapy LLLT on flap survival: measurement of flap microcirculation with Laser speckle flowmetry. Laser Ther 8:241–246CrossRef

13.

Amir A, Solomon AS, Giler S et al (2000) The influence of helium-neon laser irradiation on the viability of skin flaps in the rat. Br J Plast Surg 53:58–62PubMedCrossRef

14.

Kubota J (2002) Effects of diode laser therapy on blood flow in axial pattern flaps in the rat model. Lasers Med Sci 17:146–153PubMedCrossRef

15.

Pinfildi CE, Liebano RE, Hochman BS et al (2005)Helium-neon laser in viability of random skin flap in rats. Lasers Surg Med 37:74–77PubMedCrossRef

16.

Assis LR, Marcolino A, Pinfildi CE et al (2006) Comparison of the 904 nm and 670 nm diode lasers in the viability of random skin flap. Fisioter Bras 7:36–39

17.

Bossini PS, Fangel R, Habenschus RM et al (2009)Low-level laser therapy (670 nm) on viability of random skin flap in rats. Lasers Med Sci 24:209–213PubMedCrossRef

18.

Cury V, Bossini PS, Fangel R et al (2009) The effects of the 660 nm and 780 nm laser irradiation on viability of random skin flap in rats. Photomed Laser Surg 27:721–724PubMedCrossRef

19.

Park JB, Ahn SJ, Kang YG, Kim EC, Heo JS, Kang KL (2015) Effects of increased low-level diode laser irradiation time on extraction socket healing in rats. Lasers Med Sci 30(2):719–726PubMedCrossRef

20.

Martignago CCS, Tim CR, Assis L, Neves LMG, Bossini PS, Renno AC et al (2019) Comparison of two different laser photobiomodulation protocols on the viability of random skin flap in rats. Lasers Med Sci 34(5):1041–1047PubMedCrossRef

21.

Pugliese LS, Medrado AP, Reis SR et al (2003) The influence of low-level laser therapy on biomodulation of collagen and elastic fibers. Pesqui Odontol Bras 17:307–313PubMedCrossRef

22.

Carvalho PT, Mazzer N, Reis FA et al (2006) Analysis of the influence of low-power HeNe laser on the healing of skin wounds in diabetic and non-diabetic rats. Acta Cir Bras 21:177–183PubMedCrossRef

23.

Busnardo VL, Biondo-Simões ML (2010) Effects of low-level helium-neon laser on induced wound healing in rats. Rev Bras Fisioter 14:45–51PubMedCrossRef

24.

Prado R, Neves L, Marcolino A, Ribeiro T, Pinfildi C, Ferreira L et al (2010) Effect of low-level laser therapy on malondialdehyde concentration in random cutaneous flap viability. Photomed Laser Surg 28(3):379–384PubMedCrossRef

25.

Leite GP, das Neves LM, Silva CA, Guirro RR, de Souza TR, de Souza AK et al (2017) Photobiomodulation laser and pulsed electrical field increase the viability of the musculocutaneous flap in diabetic rats. Lasers Med Sci 32(3):641–648PubMedCrossRef

26.

Cook-Mills JM, Deem TL (2005) Active participation of endothelial cells in inflammation. J Leukoc Biol 77(4):487–495PubMedCrossRef

27.

Pinfildi CE, Liebano RE, Hochman BS, Enokihara MM, Lippert R, Gobbato RC et al (2009) Effect of low-level laser therapy on mast cells in viability of the transverse rectus abdominis musculocutaneous flap. Photomed Laser Surg 27(2):337–343PubMedCrossRef

28.

Costa MS, Pinfildi CE, Gomes HC, Liebano RE, Arias VE, Silveira TS et al (2010) Effect of low-level laser therapy with output power of 30 mW and 60 mW in the viability of a random skin flap. Photomed Laser Surg 28(1):57–61PubMedCrossRef

29.

Cury V et al (2013) Low level laser therapy increases angiogenesis in a modelo f ischemic skin flap in rats mediated by VEGF, HIF-1a and MMP-2. J Photochem Photobiol B Biol 125:164–170CrossRef

30.

Esteves Junior I, Masson IB, Oshima CT, Paiotti AP, Liebano RE, Plapler H (2012)Low-level laser irradiation, cyclooxygenase-2(COX-2) expression and necrosis of random skin flaps in rats. Lasers Med Sci 27(3):655–660PubMedCrossRef

31.

Nishioka MA, Pinfildi CE, Sheliga TR, Arias VE, Gomes HC, Ferreira LM (2012) LED (660 nm) and laser (670 nm) use on skin flap viability: angiogenesis and mast cells on transition line. Lasers Med Sci 27:1045–1050PubMedCrossRef

32.

Prado RP, Garcia SB, Thomazini JA, Piccinato CE (2012) Effects of 830 and 670 nm laser on viability of random skin flap in rats. Photomed Laser Surg 30:418–424PubMedCrossRef

33.

Pinfildi CE, Hochman BS, Nishioka MA et al (2013) What is better in TRAm Flap survival: LLLT single or multiirraddiation? Lasers Med Sci 28:755–761PubMedCrossRef

34.

Bezuhly M, Morris SF, Juskevicius R, Currie RW, West KA, Liwski RS (2009) Activated protein C improves ischemic flap survival and modulates proangiogenic and antiinflammatory gene expression. Plast Reconstr Surg 123(2):502–515PubMedCrossRef

35.

Duarte IS, Gomes HFC, Ferreira LM (1998) Effect of dimethyl sulphoxide on necrosis of skin flaps in rats. Can J Plast Surg 6(2):93–97CrossRef

36.

Davis RE, Wachholz JH, Jassir D, Perlyn CA, Agrama MH (1999) Comparison of topical anti-ischemic agents in the salvage of failing random-pattern skin flaps in rats. Arch Facial Plast Surg 1(1):27–32PubMedCrossRef

37.

Damy SB et al (2010) Aspectos fundamentais da experimentação animal – aplicações em cirurgia experimental. Rev Assoc Med Bras 56(1):103–111PubMedCrossRef

38.

McFarlane RM, Deyoung G, Henry RA (1965) The design of a pedicle flap in the rat to study necrosis and its prevention. Plast Reconstr Surg 35:177–182PubMedCrossRef

39.

Sasaki GH, Pang CY (1980) Hemodynamics and viability of acute neurovascular island skin flaps in rats. Plast Reconstr Surg 65(2):152–158PubMedCrossRef

40.

Kashimura T, Soejima K, Asami T et al (2016) The effect of mature adipocyte-derived dedifferentiated fat (dfat) cells on a dorsal skin flap model. J Investig Surg 29:6–12CrossRef

41.

Prado RP, Pinfildi CE, Liebano RE, Hochman B, Ferreira LM (2005) Diode Laser in viability of random skin flap in rats. Photomed Laser Surg 23(1):115

42.

Boschi ES, Leite CE, Saciura VC, Caberlon E, Lunardelli A, Bitencourt S et al (2008)Anti-inflammatory effects of low-level laser therapy (660 nm) in the early phase in carrageenan-induced pleurisy in rat. Lasers Surg Med 40(7):500–508PubMedCrossRef

43.

Albertini R, Aimbire F, Villaverde AB, Silva JA Jr, Costa MS (2007)COX-2 mRNA expression decreases in the subplantar muscle of rat paw subjected to carrageenan-induced inflammation after low level laser therapy. Inflamm Res 56(6):228–229PubMedCrossRef

44.

Mesquita-Ferrari RA, Martins MD, Silva JA Jr, da Silva TD, Piovesan RF, Pavesi VC et al (2010) Effects of low-level laser therapy on expression of TNF-alpha and TGF-beta in skeletal muscle during the repair process. Lasers Med Sci 26(3):335–340PubMedCrossRef

Title: Photobiomodulation effect in tumoral necrosis factor-alpha(TNF-α) on the viability of random skin flap in rats
Authors: Georgia Rodrigues Esteves
Ivaldo Esteves Junior
Igor Fagioli Bordello Masson
Aline Fernanda Perez Machado
Maria Carolina Derencio Oliveira
Cristiano Schiavinato Baldan
Thiago Saikali Farcic
Richard Eloin Liebano
Hélio Plapler
Publication date: 01-04-2022
Publisher: Springer London
Keyword: Laser
Published in: Lasers in Medical Science / Issue 3/2022
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03303-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Photobiomodulation effect in tumoral necrosis factor-alpha(TNF-α) on the viability of random skin flap in rats

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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