Top

Published in:

01-04-2022 | Laser | Original Article

New evidence from hyperspectral imaging analysis on the effect of photobiomodulation therapy on normal skin oxygenation

Authors: Mihaela Antonina Calin, Adrian Macovei, Roxana Savastru, Adriana Sarah Nica, Sorin Viorel Parasca

Published in: Lasers in Medical Science | Issue 3/2022

Abstract

The aim of this study was to assess the changes induced by photobiomodulation therapy in oxygenation of normal skin and underlying tissue using hyperspectral imaging combined with a chemometric regression approach. Eleven healthy adult volunteers were enrolled in this study. The dorsal side of the left hand of each subject was exposed to photobiomodulation therapy, while the correspondent side of the right hand was used as a control (placebo effect). Laser irradiation was performed with a laser diode system (635 nm, 15mW, 9 J/cm²) for 900 s. Changes in skin oxygenation were assessed before and after applying the photobiomodulation therapy and placebo using the hyperspectral imaging. Hyperspectral data analysis showed that variations of oxyhemoglobin and deoxyhemoglobin concentrations had no statistical significance in both groups. In conclusion, photobiomodulation therapy does not induce changes in oxyhemoglobin and deoxyhemoglobin concentrations in the normal skin measured from hyperspectral images, at least at λ = 635 nm and 900-s exposure time.

Chung H, Dai T, Sharma SK et al (2012) The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 40:516–533. https://doi.org/10.1007/s10439-011-0454-7CrossRefPubMed

Cotler HB (2015) The use of low level laser therapy (LLLT) for musculoskeletal pain. MOJ Orthop Rheumatol. https://doi.org/10.15406/mojor.2015.02.00068

Yadav A, Gupta A (2017) Noninvasive red and near-infrared wavelength-induced photobiomodulation: promoting impaired cutaneous wound healing. Photodermatol Photoimmunol Photomed 33:4–13. https://doi.org/10.1111/phpp.12282CrossRefPubMed

Dompe C, Moncrieff L, Matys J et al (2020) Photobiomodulation—underlying mechanism and clinical applications. J Clin Med 9:1724. https://doi.org/10.3390/jcm9061724CrossRefPubMedCentral

Anders JJ, Lanzafame RJ, Arany PR (2015) Low-level light/laser therapy versus photobiomodulation therapy. Photomed Laser Surg 33:183–184CrossRef

De Freitas LF, Hamblin MR (2016) Proposed mechanisms of photobiomodulation or low-level light therapy. IEEE J Sel Top Quantum Electron 22:348–364. https://doi.org/10.1109/JSTQE.2016.2561201CrossRef

Yamany AA, Sayed HM (2012) Effect of low level laser therapy on neurovascular function of diabetic peripheral neuropathy. J Adv Res 3:21–28. https://doi.org/10.1016/j.jare.2011.02.009CrossRef

Frangez I, Cankar K, Ban Frangez H, Smrke DM (2017) The effect of LED on blood microcirculation during chronic wound healing in diabetic and non-diabetic patients—a prospective, double-blind randomized study. Lasers Med Sci 32:887–894. https://doi.org/10.1007/s10103-017-2189-7CrossRefPubMed

Linares SN, Beltrame T, Ferraresi C et al (2020) Photobiomodulation effect on local hemoglobin concentration assessed by near-infrared spectroscopy in humans. Lasers Med Sci 35:641–649. https://doi.org/10.1007/s10103-019-02861-xCrossRefPubMed

10.

Wang X, Tian F, Soni SS, et al (2016) Interplay between up-regulation of cytochrome-c-oxidase and hemoglobin oxygenation induced by near-infrared laser. Sci Rep. https://doi.org/10.1038/srep30540

11.

Heu F, Forster C, Namer B et al (2013) Effect of low-level laser therapy on blood flow and oxygen- hemoglobin saturation of the foot skin in healthy subjects: a pilot study. Laser Ther 22:21–30. https://doi.org/10.5978/islsm.13-OR-03CrossRefPubMedPubMedCentral

12.

Rogatkin DA, Dunaev AV (2014) Stimulation of blood microcirculation at low level laser therapy: Monitoring tools and preliminary data. Int J Med Res Dev 3(1):100–106

13.

Goetz AFH (2009) Three decades of hyperspectral remote sensing of the Earth: a personal view. Remote Sens Environ 113:S5–S16. https://doi.org/10.1016/j.rse.2007.12.014CrossRef

14.

Goetz AFH, Vane G, Solomon JE, Rock BN (1985) Imaging spectrometry for earth remote sensing. Science (80- ) 228:1147–1153. https://doi.org/10.1126/science.228.4704.1147

15.

Zhang Y, Wu X, He L et al (2020) Applications of hyperspectral imaging in the detection and diagnosis of solid tumors. Transl Cancer Res 9:1265–1277. https://doi.org/10.21037/tcr.2019.12.53CrossRefPubMedPubMedCentral

16.

Paluchowski LA, Nordgaard HB, Bjorgan A et al (2016) Can spectral–spatial image segmentation be used to discriminate experimental burn wounds? J Biomed Opt 21:101413. https://doi.org/10.1117/1.jbo.21.10.101413CrossRefPubMed

17.

Parasca SV, Calin MA, Manea D et al (2018) Hyperspectral index-based metric for burn depth assessment. Biomed Opt Express 9:5778. https://doi.org/10.1364/boe.9.005778CrossRefPubMedPubMedCentral

18.

Yang Q, Sun S, Jeffcoate W et al (2018) Investigation of the performance of hyperspectral imaging by principal component analysis in the prediction of healing of diabetic foot ulcers. J Imaging 4:144. https://doi.org/10.3390/jimaging4120144CrossRef

19.

Chiang N, Jain JK, Sleigh J, Vasudevan T (2017) Evaluation of hyperspectral imaging technology in patients with peripheral vascular disease. In: Journal of Vascular Surgery. Mosby Inc., pp 1192–1201

20.

Liu N, Guo Y, Jiang H, Yi W (2020) Gastric cancer diagnosis using hyperspectral imaging with principal component analysis and spectral angle mapper. J Biomed Opt 25:1. https://doi.org/10.1117/1.JBO.25.6.066005CrossRefPubMed

21.

Fabelo H, Ortega S, Ravi D et al (2018) Spatio-spectral classification of hyperspectral images for brain cancer detection during surgical operations. PLoS ONE 13:e0193721. https://doi.org/10.1371/journal.pone.0193721CrossRefPubMedPubMedCentral

22.

Halicek M, Fabelo H, Ortega S et al (2019) Hyperspectral imaging for head and neck cancer detection: specular glare and variance of the tumor margin in surgical specimens. J Med Imaging 6:1. https://doi.org/10.1117/1.jmi.6.3.035004CrossRef

23.

Li Y, Xie X, Yang X, et al (2019) Diagnosis of early gastric cancer based on fluorescence hyperspectral imaging technology combined with partial-least-square discriminant analysis and support vector machine. J Biophotonics. https://doi.org/10.1002/jbio.201800324

24.

Torti E, Florimbi G, Castelli F et al (2018) Parallel K-means clustering for brain cancer detection using hyperspectral images. Electronics 7:283. https://doi.org/10.3390/electronics7110283CrossRef

25.

Denstedt M, Pukstad BS, Paluchowski LA, et al (2013) Hyperspectral imaging as a diagnostic tool for chronic skin ulcers. In: Kollias N, Choi B, Zeng H, et al (eds) Photonic Therapeutics and Diagnostics IX. SPIE, p 85650N

26.

Carmona Jaramillo D, Escobar JE, Galeano J, Torres-Madronero MC (2020) Design of a multilayer neural network for the classification of skin ulcers’ hyperspectral images: a proof of concept. In: Brieva J, Romero E, Lepore N (eds) 15th International Symposium on Medical Information Processing and Analysis. SPIE, p 14

27.

Chen YM, Lai KL, Chen HH et al (2020) Hyperspectral imaging for skin assessment in systemic sclerosis: a pilot study. Revmatol 59:3201–3210. https://doi.org/10.1093/rheumatology/keaa067CrossRef

28.

Sassaroli A, Fantini S (2004) Comment on the modified Beer-Lambert law for scattering media. Phys Med Biol 49

29.

Moré JJ (1978) The Levenberg-Marquardt algorithm: implementation and theory. Springer, Berlin, Heidelberg, pp 105–116

30.

Polder G, van der Heijden GWAM, Keizer LCP, Young IT (2003) Calibration and characterisation of imaging spectrographs. J Near Infrared Spectrosc 11:193–210. https://doi.org/10.1255/jnirs.366CrossRef

31.

Green AA, Berman M, Switzer P, Craig MD (1988) A transformation for ordering multispectral data in terms of image quality with implications for noise removal. IEEE Trans Geosci Remote Sens 26:65–74. https://doi.org/10.1109/36.3001CrossRef

32.

Miclos S, Parasca SV, Calin MA et al (2015) Algorithm for mapping cutaneous tissue oxygen concentration using hyperspectral imaging. Biomed Opt Express 6:3420. https://doi.org/10.1364/boe.6.003420CrossRefPubMedPubMedCentral

33.

Mishra P, Pandey CM, Singh U et al (2019) Descriptive statistics and normality tests for statistical data. Ann Card Anaesth 22:67–72. https://doi.org/10.4103/aca.ACA_157_18CrossRefPubMedPubMedCentral

34.

Khaodhiar L, Dinh T, Schomacker KT et al (2007) The use of medical hyperspectral technology to evaluate microcirculatory changes in diabetic foot ulcers and to predict clinical outcomes. Diabetes Care 30:903–910. https://doi.org/10.2337/dc06-2209CrossRefPubMed

35.

Calin MA, Boiangiu IC, Parasca SV et al (2017) Blood oxygenation monitoring using hyperspectral imaging after flap surgery. Spectrosc Lett 50:150–155. https://doi.org/10.1080/00387010.2017.1297957CrossRef

36.

Marotz J, Kulcke A, Siemers F, et al (2019) Extended perfusion parameter estimation from hyperspectral imaging data for bedside diagnostic in medicine. Molecules. https://doi.org/10.3390/molecules24224164

37.

Calin MA, Macovei A, Miclos S et al (2017) Simulated altitude exposure assessment by hyperspectral imaging. J Biomed Opt 22:056012. https://doi.org/10.1117/1.jbo.22.5.056012CrossRef

38.

Chin MS (2012) Hyperspectral imaging for early detection of oxygenation and perfusion changes in irradiated skin. J Biomed Opt 17:026010. https://doi.org/10.1117/1.jbo.17.2.026010CrossRefPubMed

Title: New evidence from hyperspectral imaging analysis on the effect of photobiomodulation therapy on normal skin oxygenation
Authors: Mihaela Antonina Calin
Adrian Macovei
Roxana Savastru
Adriana Sarah Nica
Sorin Viorel Parasca
Publication date: 01-04-2022
Publisher: Springer London
Keyword: Laser
Published in: Lasers in Medical Science / Issue 3/2022
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03397-9

At a glance: The STEP trials

Springer Medicine

New evidence from hyperspectral imaging analysis on the effect of photobiomodulation therapy on normal skin oxygenation

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2022

Photobiomodulation therapy upregulates the growth kinetics and multilineage differentiation potential of human dental pulp stem cells—an in vitro Study

Laser-assisted hatching zona thinning does not improve the pregnancy outcomes of poor-quality blastocysts in frozen-thawed embryo transfer cycle: a retrospective cohort study

Effects of multiple modes of UltraPulse fractional CO2 laser treatment on extensive scarring: a retrospective study

Laterally closed tunnel technique with and without adjunctive photobiomodulation therapy for the management of isolated gingival recession—a randomized controlled assessor-blinded clinical trial

Antidepressant and antioxidant effects of transcranial irradiation with 830-nm low-power laser in an animal model of depression

Excimer laser atherectomy combined with drug-coated balloon versus drug-eluting balloon angioplasty for the treatment of infrapopliteal arterial revascularization in ischemic diabetic foot: 24-month outcomes