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01-08-2021 | Laser | Original Article

Involvement of substance P, osteopontin and satellite glial cells on photobiomodulation-induced antinociceptive effect in an experimental model of dentin hypersensitivity

Authors: Victhor Teixeira de Oliveira, João Ignácio Ferrara-Jr, Heloísa Alonso Matielo, Adilson da Silva Alves, Luiz Roberto Britto, Ana Cecilia Corrêa Aranha, Camila Squarzoni Dale

Published in: Lasers in Medical Science | Issue 6/2021

Abstract

The aim of this work was to investigate the involvement of substance P (SP), osteopontin (OPN), and satellite glial cells (SGC) on photobiomodulation-induced (PBM) antinociceptive effect in an experimental model of dentin hypersensitivity (DH). Rats ingested isotonic drink (ID, pH 2.87) for 45 consecutive days and after this period received PBM irradiation at λ660 nm or λ808 nm (1 J, 3.5 J/cm², 100 mW, 10 s, 0.028 cm², continuous wave, 3 consecutive daily sessions), and were evaluated for nociceptive behavior 24, 48, 72 h, and 14 days after laser treatments. ID ingestion induced an increase on thermal sensitivity of DH characteristics in rats that was completely reversed by PBM treatment at both 660 and 808 nm. Immunohistochemical analysis revealed increased SP expression at both dentin-pulp complex (DPC) and trigeminal ganglia (TG) of DH-rats which did not occur in PBM groups by PBM treatment. Also, the increase of glial fibrillary acidic protein (GFAP) observed in the TG of DH-rats was also reversed by PBM treatment. Finally, PBM at both 660 and 808 nm increased OPN expression in the dentin-pulp complex of DH-rats after 14 days of PBM treatment. All in all, this data demonstrates that PBM reverses nociception in a DH experimental model by inhibiting neurogenic inflammation and inducing a regenerative response.

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Title: Involvement of substance P, osteopontin and satellite glial cells on photobiomodulation-induced antinociceptive effect in an experimental model of dentin hypersensitivity
Authors: Victhor Teixeira de Oliveira
João Ignácio Ferrara-Jr
Heloísa Alonso Matielo
Adilson da Silva Alves
Luiz Roberto Britto
Ana Cecilia Corrêa Aranha
Camila Squarzoni Dale
Publication date: 01-08-2021
Publisher: Springer London
Keyword: Laser
Published in: Lasers in Medical Science / Issue 6/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03246-9

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Involvement of substance P, osteopontin and satellite glial cells on photobiomodulation-induced antinociceptive effect in an experimental model of dentin hypersensitivity

Abstract

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Abstract

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