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Open Access 01-03-2010 | Original

Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury

Authors: Masahiko Katayama, Akitoshi Ishizaka, Michiie Sakamoto, Seitaro Fujishima, Kiyotoshi Sekiguchi, Koichiro Asano, Tomoko Betsuyaku, Toru Kotani, Lorraine B. Ware, Michael A. Matthay, Satoru Hashimoto

Published in: Intensive Care Medicine | Issue 3/2010

Abstract

Objective

Laminin-5, a cell adhesive molecule expressed solely by epithelium, is known to enhance epithelial cell migration and repair of injured epithelium, after its essential component γ2-chain is processed proteolytically. Our previous study revealed circulating levels of amino-terminal fragment of laminin γ2-chain (G2F) reflect epithelial tumor invasiveness in carcinoma patients, but its physiological role in alveolar epithelial injury remains unknown.

Design

Sampling of epithelial lining fluids or pulmonary edema fluids from patients with acute lung injury (ALI) or related diseases was performed. Plasma samples were obtained from them at the time of disease onset or later. G2F concentrations were determined by immunoassay constructed by ourselves.

Results

We found a significantly higher amount of G2F in pulmonary edema and epithelial lining fluids of patients with ALI, as compared with those with the other respiratory diseases. Their plasma levels were also elevated significantly early at the onset of ALI (mean ± SD; 147 ± 82 ng/ml in non-surviving and 90 ± 56 in surviving patients) as compared with those in the patients with cardiogenic pulmonary edema (59 ± 36) or idiopathic pulmonary fibrosis (37 ± 17), indicating alveolar epithelium rapidly secrete laminin-5 in ALI. At 5 days after onset, non-surviving patients maintained higher plasma concentrations (152 ± 84), but in contrast, the levels in surviving patients declined (71 ± 35), suggesting secretion of laminin-5 was suppressed, associated with recovery from ALI.

Conclusion

Circulating G2F may be a biomarker for alveolar laminin-5 secreted early at disease onset in ALI, potentially regulating alveolar re-epithelialization.

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Title: Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury
Authors: Masahiko Katayama
Akitoshi Ishizaka
Michiie Sakamoto
Seitaro Fujishima
Kiyotoshi Sekiguchi
Koichiro Asano
Tomoko Betsuyaku
Toru Kotani
Lorraine B. Ware
Michael A. Matthay
Satoru Hashimoto
Publication date: 01-03-2010
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 3/2010
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-009-1719-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury

Abstract

Objective

Design

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Design

Results

Conclusion

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