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BMC Pediatrics

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01-12-2021 | Kawasaki Disease | Research

Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease

Authors: Xue Fan, Yuhan Zhou, Xin Guo, Mingguo Xu

Published in: BMC Pediatrics | Issue 1/2021

Abstract

Background

Kawasaki disease (KD) is the main cause of acquired heart disease in children and can lead to coronary artery lesions. This present study was designed to analyze the characteristics of KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequencing (scRNA-seq) and to explore the potential molecular mechanism of KD.

Methods

PBMC was collected from one healthy child and one KD patient, and was used to single-cell RNA sequencing for cell clusters identification and differently expressed gene (DEG) determination. GO function enrichment analysis of DEG in B cell and T cells were performed to explore the most active biological function in KD immune cells.

Results

Twelve cell clusters can be identified in two samples. Compared with healthy child, naive CD8+ T cell, T helper cell and B cell in KD child were decreased, mainly immune-related T cells, and natural killer T (NKT) cell were increased. Cell activation, lymphocyte activation and regulation of immune system process were 3 GO function shared by all four types of T cells and B cell.

Conclusions

Immune cell disorder appears in the KD patient at single cell level by scRNA-seq.

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Title: Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease
Authors: Xue Fan
Yuhan Zhou
Xin Guo
Mingguo Xu
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Kawasaki Disease
Kawasaki Disease
Kawasaki Disease
Published in: BMC Pediatrics / Issue 1/2021
Electronic ISSN: 1471-2431
DOI: https://doi.org/10.1186/s12887-021-02754-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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