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BMC Physiology
Published in: BMC Physiology 1/2011

Open Access 01-12-2011 | Research article

JAK/STAT signaling and human in vitro myogenesis

Authors: Marissa K Trenerry, Paul A Della Gatta, David Cameron-Smith

Published in: BMC Physiology | Issue 1/2011

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Abstract

Background

A population of satellite cells exists in skeletal muscle. These cells are thought to be primarily responsible for postnatal muscle growth and injury-induced muscle regeneration. The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling cascade has a crucial role in regulating myogenesis. In rodent skeletal muscle, STAT3 is essential for satellite cell migration and myogenic differentiation, regulating the expression of myogenic factors. The aim of the present study was to investigate and compare the expression profile of JAK/STAT family members, using cultured primary human skeletal muscle cells.

Results

Near confluent proliferating myoblasts were induced to differentiate for 1, 5 or 10 days. During these developmental stages, members of the JAK/STAT family were examined, along with factors known to regulate myogenesis. We demonstrate the phosphorylation of JAK1 and STAT1 only during myoblast proliferation, while JAK2 and STAT3 phosphorylation increases during differentiation. These increases were correlated with the upregulation of genes associated with muscle maturation and hypertrophy.

Conclusions

Taken together, these results provide insight into JAK/STAT signaling in human skeletal muscle development, and confirm recent observations in rodents.
Appendix
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Metadata
Title
JAK/STAT signaling and human in vitro myogenesis
Authors
Marissa K Trenerry
Paul A Della Gatta
David Cameron-Smith
Publication date
01-12-2011
Publisher
BioMed Central
Published in
BMC Physiology / Issue 1/2011
Electronic ISSN: 1472-6793
DOI
https://doi.org/10.1186/1472-6793-11-6

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