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Virology Journal
Published in: Virology Journal 1/2011

Open Access 01-12-2011 | Research

Isolation and characterization of two new hepatitis E virus genotype 1 strains from two mini-outbreaks in Lahore, Pakistan

Authors: Tahir Iqbal, Muhammad Idrees, Liaqat Ali, Abrar Hussain, Muhammad Ali, Sadia Butt, Muhammad Zubair Yousaf, Muhammad Farooq Sabar

Published in: Virology Journal | Issue 1/2011

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Abstract

Background

Pakistan is a highly endemic area for hepatitis E virus (HEV) infection. The aim of the current study was to isolate and characterize strains of HEV in two mini outbreaks.

Results

RNA was extracted and reverse transcribed to cDNA. Nested PCR was done for the detection of HEV RNA. The positive bands were eluted, cloned in TA vector and sequenced in both directions using genetic Analyzer (Applied Biosystem Inc, USA). Phylogenetic analysis was done using MEGA4 software. We isolated two new HEV genotype-1 strains from Lahore, Pakistan, based on cloning and sequencing of ORF2 region.

Conclusions

Our study suggest that both the human HEV strains were closely related to the Sar-55 but different from the Abb-2B and 87-Pakistan-B HEV isolates sharing 88-91% sequence identity to Pakistani isolate Sar-55. These results indicated that Sar-55 is the main endemic HEV strain in various areas of the country.
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Literature
1.
Balayan MS: Epidemiology of hepatitis E virus infection. J Viral Hepat 1997, 4: 155-165. 10.1046/j.1365-2893.1997.00145.xCrossRefPubMed
2.
Tsarev SA, Emerson SU, Reyes GR, Tsareva TS, Legters LJ, Malik IA, Iqbal M, Purcell RH: Characterization of a prototype strain of hepatitis E virus. Proc Natl Acad Sci USA 1992, 89: 559-563. 10.1073/pnas.89.2.559PubMedCentralCrossRefPubMed
3.
Ticehurst J, Rhodes LL, Krawczynski K, Asher LV, Engler WF, Mensing TL, Caudill JD, Sjogren MH, Hoke CH, LeDuc JW, et al.: Infection of owl monkeys (Aotus trivirgatus) and cynomolgus monkeys (Macaca fascicularis) with hepatitis E virus from Mexico. J Infect Dis 1992, 165: 835-845.CrossRefPubMed
4.
Meng XJ: Swine hepatitis E virus: cross-species infection and risk in xenotransplantation. Curr Top Microbiol Immunol 2003, 278: 185-216.PubMed
5.
Viswanathan R, Sidhu AS: Infectious hepatitis; clinical findings. Indian J Med Res 1957, 45: 49-58.PubMed
6.
Khuroo MS: Study of an epidemic of non-A, non-B hepatitis. Possibility of another human hepatitis virus distinct from post-transfusion non-A, non-B type. Am J Med 1980, 68: 818-824. 10.1016/0002-9343(80)90200-4CrossRefPubMed
7.
Huang CC, Nguyen D, Fernandez J, Yun KY, Fry KE, Bradley DW, Tam AW, Reyes GR: Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology 1992, 191: 550-558. 10.1016/0042-6822(92)90230-MCrossRefPubMed
8.
Tam AW, Smith MM, Guerra ME, Huang CC, Bradley DW, Fry KE, Reyes GR: Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 1991, 185: 120-131. 10.1016/0042-6822(91)90760-9CrossRefPubMed
9.
Worm HC, Schlauder GG, Brandstatter G: Hepatitis E and its emergence in non-endemic areas. Wien Klin Wochenschr 2002, 114: 663-670.PubMed
10.
Aggarwal R, Kini D, Sofat S, Naik SR, Krawczynski K: Duration of viraemia and faecal viral excretion in acute hepatitis E. Lancet 2000, 356: 1081-1082. 10.1016/S0140-6736(00)02737-9CrossRefPubMed
11.
Zafrullah M, Ozdener MH, Panda SK, Jameel S: The ORF3 protein of hepatitis E virus is a phosphoprotein that associates with the cytoskeleton. J Virol 1997, 71: 9045-9053.PubMedCentralPubMed
12.
13.
He J: Molecular detection and sequence analysis of a new hepatitis E virus isolate from Pakistan. J Viral Hepat 2006, 13: 840-844. 10.1111/j.1365-2893.2006.00774.xCrossRefPubMed
14.
Rab MA, Bile MK, Mubarik MM, Asghar H, Sami Z, Siddiqi S, Dil AS, Barzgar MA, Chaudhry MA, Burney MI: Water-borne hepatitis E virus epidemic in Islamabad, Pakistan: a common source outbreak traced to the malfunction of a modern water treatment plant. Am J Trop Med Hyg 1997, 57: 151-157.PubMed
Metadata
Title
Isolation and characterization of two new hepatitis E virus genotype 1 strains from two mini-outbreaks in Lahore, Pakistan
Authors
Tahir Iqbal
Muhammad Idrees
Liaqat Ali
Abrar Hussain
Muhammad Ali
Sadia Butt
Muhammad Zubair Yousaf
Muhammad Farooq Sabar
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-8-94

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