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Journal of Orthopaedic Science

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01-01-2013 | Original Article

Isolation and characterization of endothelial cells from intramuscular hemangioma

Authors: Jae Hwan Cho, Ilkyu Han, Mi Ra Lee, Hwan Seong Cho, Joo Han Oh, Han Soo Kim

Published in: Journal of Orthopaedic Science | Issue 1/2013

Abstract

Background

Intramuscular hemangiomas (IMHs) are benign vascular tumors of deep soft tissue characterized by endothelial cell (EC) proliferation. The purpose of this study was to isolate ECs from IMH, characterize their angiogenic phenotype and functional characteristics, and search for a possible signaling pathway related to IMH development.

Methods

EC Isolation from IMH was performed by digestion, filtration, washing, incubation, and purification in sequence. Tie2 expression was compared between ECs from IMH and controls using reverse transcriptase polymerase chain reaction (RT-PCR). Cell invasion and proliferation assays were used to analyze functional responses of ECs to angiopoietin 1 (Ang1) and vascular endothelial growth factor (VEGF). Expression of downstream targets was analyzed using Western blot analysis.

Results

Isolated ECs showed typical cobblestone appearance under light microscopy and formed capillary-like tubular structures using Matrigel tube-forming assay. RT-PCR of isolated ECs from six patients showed increased expression of Tie2 and VEGF receptor 1 (VEGFR1) compared with control ECs. Tie2 activation by Ang1 compared with VEGFR1 by VEGF resulted in increased EC migration and proliferation. Western blot analysis showed increased Tie2 expression in hemangioma samples compared with normal ECs. Phosphorylated Akt and phosphorylated forkhead box O1 (FOXO1) expression was observed in hemangioma samples only.

Conclusion

EC isolation from IMH could be a useful tool for further research. These results suggest that increased Tie2 expression, via Akt-FOXO1 pathway activation, may play an important role in IMH pathogenesis.

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Title: Isolation and characterization of endothelial cells from intramuscular hemangioma
Authors: Jae Hwan Cho
Ilkyu Han
Mi Ra Lee
Hwan Seong Cho
Joo Han Oh
Han Soo Kim
Publication date: 01-01-2013
Publisher: Springer Japan
Published in: Journal of Orthopaedic Science / Issue 1/2013
Print ISSN: 0949-2658
Electronic ISSN: 1436-2023
DOI: https://doi.org/10.1007/s00776-012-0303-z

At a glance: The STEP trials

Springer Medicine

Isolation and characterization of endothelial cells from intramuscular hemangioma

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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