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BMC Complementary Medicine and Therapies

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Open Access 01-12-2020 | Research article

Isatis root polysaccharide promotes maturation and secretory function of monocyte-derived dendritic cells

Authors: Xuebing Wang, Zewen Chen, Tong Chen, Xiao Li, Shucheng Huang, Hao Wang, Chao Tong, Fang Liu

Published in: BMC Complementary Medicine and Therapies | Issue 1/2020

Abstract

Background

Pseudorabies virus (PRV) is an animal virus that is globally responsible for the high economic losses in the swine industry. Isatis root is a traditional Chinese medicinal herb that possesses immune-enhancing and antiviral properties. However, the molecular mechanisms underlying the effects of the active component of the isatis root polysaccharide (IRPS) extract on immature dendritic cells remain elusive.

Methods

In this study, we investigated the molecular changes in primary porcine peripheral blood monocyte-derived dendritic cells (MoDCs) during PRV infection, using enzyme-linked immunosorbent assay (ELISA) and quantitative reverse transcription-polymerase chain reaction. Additionally, we studied the effect of IRPS on PRV-infected DCs.

Results

The results showed that IRPS stimulated the maturation of MoDCs, induced IL-12 secretion, and downregulated IL-6 expression.

Conclusions

Collectively, these results suggest that IRPS is a promising candidate for promoting maturation of DCs and enhancing their secretory potential after PRV infection.

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Title: Isatis root polysaccharide promotes maturation and secretory function of monocyte-derived dendritic cells
Authors: Xuebing Wang
Zewen Chen
Tong Chen
Xiao Li
Shucheng Huang
Hao Wang
Chao Tong
Fang Liu
Publication date: 01-12-2020
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2020
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-020-03103-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Isatis root polysaccharide promotes maturation and secretory function of monocyte-derived dendritic cells

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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