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International Urogynecology Journal

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01-12-2009 | Original Article

Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?

Authors: Weng Chi Man, Jason Yen-Ping Ho, Yan Wen, Eric R. Sokol, Mary L. Polan, Bertha Chen

Published in: International Urogynecology Journal | Issue 12/2009

Abstract

Introduction and hypothesis

We investigated whether the expression of alpha-1 antitrypsin (ATT), neutrophil elastase (NE), and lysyl oxidase-like protein 1 (LOXL-1) vary within the vagina in subjects with pelvic organ prolapse (POP).

Methods

Biopsies were obtained from the anterior and posterior vaginal wall of 22 women with POP (≥stage 2 by POP-Q). The subjects were grouped by the most prominent defect: cystocele, cystocele plus uterine prolapse, and rectocele. Comparative real-time PCR, Western blotting, and NE enzyme activity assay were performed.

Results

The ratio of anterior and posterior vaginal wall ATT, NE, and LOXL-1 expression varied between individuals within the same defect group.

Conclusions

ATT, NE, and LOXl-1 expression was variable among different biopsy sites in the vagina. No consistent pattern was present when the subjects were grouped by the most prominent defect. We recommend careful consideration of biopsy sites in future studies on POP to enhance reproducibility of data.

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Title: Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?
Authors: Weng Chi Man
Jason Yen-Ping Ho
Yan Wen
Eric R. Sokol
Mary L. Polan
Bertha Chen
Publication date: 01-12-2009
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 12/2009
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-009-0905-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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