Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Cancer
Published in: BMC Cancer 1/2005

Open Access 01-12-2005 | Research article

Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma

Authors: Yen-Ching Lee, Janeanne R Perren, Evelyn L Douglas, Michael P Raynor, Maria A Bartley, Peter G Bardy, Sally-Anne Stephenson

Published in: BMC Cancer | Issue 1/2005

Login to get access

Abstract

Background

The EphB4 receptor tyrosine kinase has been reported as increased in tumours originating from several different tissues and its expression in a prostate cancer xenograft model has been reported.

Methods

RT-PCR, western blotting and immunohistochemical techniques were used to examine EphB4 expression and protein levels in human prostate cancer cell lines LNCaP, DU145 and PC3. Immunohistochemistry was also used to examine localisation of EphB4 in tissue samples from 15 patients with prostate carcinomas.

Results

All three prostate cancer cell lines expressed the EphB4 gene and protein. EphB4 immunoreactivity in vivo was significantly greater in human prostate cancers as compared with matched normal prostate epithelium and there appeared to be a trend towards increased expression with higher grade disease.

Conclusion

EphB4 is expressed in prostate cancer cell lines with increased expression in human prostate cancers when compared with matched normal tissue. EphB4 may therefore be a useful anti-prostate cancer target.
Appendix
Available only for authorised users
Literature
1.
Zwick E, Bange J, Ullrich A: Receptor tyrosine kinase signalling as a target for cancer intervention strategies. Endocr Relat Cancer. 2001, 8: 161-73. 10.1677/erc.0.0080161.CrossRefPubMed
2.
Zwick E, Bange J, Ullrich A: Receptor tyrosine kinases as targets for anticancer drugs. Trends Mol Med. 2002, 8 (1): 17-23. 10.1016/S1471-4914(01)02217-1.CrossRefPubMed
3.
Himanen JP, Nikolov DB: Eph receptors and ephrins. Int J Biochem Cell Biol. 2003, 35: 130-4. 10.1016/S1357-2725(02)00096-1.CrossRefPubMed
4.
Eph Nomenclature Committee: Unified nomenclature for Eph family receptors and their ligands, the ephrins. Cell. 1997, 90: 403-4. 10.1016/S0092-8674(00)80500-0.CrossRef
5.
Pasquale EB: Eph receptors signalling casts a wide net on cell behaviour. Nat Rev Mol Cell Biol. 2005, 6: 462-75. 10.1038/nrm1662.CrossRefPubMed
6.
Bruckner K, Klein R: Signalling by Eph receptors and their ephrin ligands. Curr Opin Neurobiol. 1998, 8: 375-82. 10.1016/S0959-4388(98)80064-0.CrossRefPubMed
7.
Adams RH, Klein R: Eph receptors and ephrin ligands. Essential mediators of vascular development. Trends Cardiovasc Med. 2000, 10: 183-8. 10.1016/S1050-1738(00)00046-3.CrossRefPubMed
8.
Wang HU, Chen ZF, Anderson DJ: Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 1998, 93: 741-53. 10.1016/S0092-8674(00)81436-1.CrossRefPubMed
9.
Nakamoto M, Bergemann AD: Diverse roles for the Eph family of receptor tyrosine kinases in carcinogenesis. Microsc Res Tech. 2002, 59: 58-67. 10.1002/jemt.10177.CrossRefPubMed
10.
Bennett BD, Wang Z, Kuang WJ, Wang A, Groopman JE, Goeddel DV, Scadden DT: Cloning and characterization of HTK, a novel transmembrane tyrosine kinase of the EPH subfamily. J Biol Chem. 1994, 269: 14211-8.PubMed
11.
Stephenson SA, Slomka S, Douglas EL, Hewett PJ, Hardingham JE: Receptor protein tyrosine kinase EphB4 is up-regulated in colon cancer. BMC Mol Biol. 2001, 2: 15-10.1186/1471-2199-2-15.CrossRefPubMedPubMedCentral
12.
Liu W, Ahmad SA, Jung YD, Reinmuth N, Fan F, Bucana CD, Ellis LM: Coexpression of ephrin-Bs and their receptors in colon carcinoma. Cancer. 2002, 94: 934-9. 10.1002/cncr.10122.CrossRefPubMed
13.
Berclaz G, Andres AC, Albrecht D, Dreher E, Ziemiecki A, Gusterson BA, Crompton MR: Expression of the receptor protein tyrosine kinase myk-1/htk in normal and malignant mammary epithelium. Biochem Biophys Res Commun. 1996, 226: 869-75. 10.1006/bbrc.1996.1442.CrossRefPubMed
14.
Wu Q, Suo Z, Risberg B, Karlsson MG, Villman K, Nesland JM: Expression of Ephb2 and Ephb4 in breast carcinoma. Pathol Oncol Res. 2004, 10: 26-33.CrossRefPubMed
15.
Takai N, Miyazaki T, Fujisawa K, Nasu K, Miyakawa I: Expression of receptor tyrosine kinase EphB4 and its ligand ephrin-B2 is associated with malignant potential in endometrial cancer. Oncol Rep. 2001, 8: 567-73.PubMed
16.
Berclaz G, Karamitopoulou E, Mazzucchelli L, Rohrbach V, Dreher E, Ziemiecki A, Andres AC: Activation of the receptor protein tyrosine kinase EphB4 in endometrial hyperplasia and endometrial carcinoma. Ann Oncol. 2003, 14: 220-6. 10.1093/annonc/mdg072.CrossRefPubMed
17.
Tang XX, Brodeur GM, Campling BG, Ikegaki N: Coexpression of transcripts encoding EPHB receptor protein tyrosine kinases and their ephrin-B ligands in human small cell lung carcinoma. Clin Cancer Res. 1999, 5: 455-60.PubMed
18.
Sinha UK, Kundra A, Scalia P, Smith DL, Parsa B, Masood R, Gill PS: Expression of EphB4 in head and neck squamous cell carcinoma. Ear Nose Throat J. 2003, 82: 866-887.PubMed
19.
Cromer A, Carles A, Millon R, Ganguli G, Chalmel F, Lemaire F, Young J, Dembele D, Thibault C, Muller D, Poch O, Abecassis J, Wasylyk B: Identification of genes associated with tumorigenesis and metastatic potential of hypopharyngeal cancer by microarray analysis. Oncogene. 2004, 23: 2484-98. 10.1038/sj.onc.1207345.CrossRefPubMed
20.
Robinson D, He F, Pretlow T, Kung HJ: A tyrosine kinase profile of prostate carcinoma. Proc Natl Acad Sci U S A. 1996, 93: 5958-62. 10.1073/pnas.93.12.5958.CrossRefPubMedPubMedCentral
21.
Xia G, Kumar SR, Masood R, Zhu S, Reddy R, Krasnoperov V, Quinn DI, Henshell SM, Sutherland RL, Pinski JK, Daneshmand S, Buscarini M, Stein JP, Zhong C, Broek D, Roy-Burman P, Gill PS: EphB4 expression and biological significance in prostate cancer. Cancer Res. 2005, 65: 4623-32. 10.1158/0008-5472.CAN-04-2667.CrossRefPubMed
22.
Horoszewicz JS, Leong SS, Kawinski E, Karr JP, Rosenthal H, Chu TM, Mirand EA, Murphy GP: LNCaP model of human prostatic carcinoma. Cancer Res. 1983, 43: 1809-18.PubMed
23.
Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF: Isolation of a human prostate carcinoma cell line (DU 145). Int J Cancer. 1978, 21: 274-81.CrossRefPubMed
24.
Kaighn ME, Narayan KS, Ohnuki Y, Lechner JF, Jones LW: Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest Urol. 1979, 17: 16-23.PubMed
25.
Sakano S, Serizawa R, Inada T, Iwama A, Itoh A, Kato C, Shimizu Y, Shinkai F, Shimizu R, Kondo S, Ohno M, Suda T: Characterisation of a ligand for receptor protein-tyrosine kinase HTK expressed in immature haematopoietic cells. Oncogene. 1996, 13: 813-22.PubMed
26.
Nikolova Z, Djonov V, Zuercher G, Andres AC, Ziemiecki A: Cell-type specific and estrogen dependent expression of the receptor tyrosine kinase EphB4 and its ligand ephrin-B2 during mammary gland morphogenesis. J Cell Sci. 1998, 111: 2741-51.PubMed
27.
Berclaz G, Flutsch B, Altermatt HJ, Rohrbach V, Djonov V, Ziemiecki A, Dreher E, Andres AC: Loss of EphB4 receptor tyrosine kinase protein expression during carcinogenesis of the human breast. Oncol Rep. 2002, 9: 985-9.PubMed
28.
Nakada M, Niska JA, Miyamori H, McDonough WS, Wu J, Sato H, Berens ME: The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells. Cancer Res. 2004, 64: 3179-85. 10.1158/0008-5472.CAN-03-3667.CrossRefPubMed
29.
Zelinski DP, Zantek ND, Stewart JC, Irizarry AR, Kinch MS: EphA2 overexpression causes tumorigenesis of mammary epithelial cells. Cancer Res. 2001, 61: 2301-6.PubMed
30.
Maru Y, Hirai H, Yoshida MC, Takaku F: Evolution, expression and chromosomal location of a novel receptor tyrosine kinase gene, eph. Mol Cell Biol. 1988, 8 (9): 3770-76.CrossRefPubMedPubMedCentral
31.
Munarini N, Jager R, Abderhalden S, Zuercher G, Rohrbach V, Loercher S, Pfanner-Meyer B, Andres AC, Ziemiecki A: Altered mammary epithelial development, pattern formation and involution in transgenic mice expressing the EphB4 receptor tyrosine kinase. J Cell Sci. 2002, 115: 25-37.PubMed
32.
Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG: Inhibition of tumor growth and angiogenesis by soluble EphB4. Neoplasia. 2004, 6: 248-57.CrossRefPubMedPubMedCentral
33.
Oates AC, Lackmann M, Power MA, Brennan C, Down LM, Do C, Evans B, Holder N, Boyd AW: An early developmental role for eph-ephrin interaction during vertebrate gastrulation. Mech Dev. 1999, 83: 77-94. 10.1016/S0925-4773(99)00036-2.CrossRefPubMed
34.
O'Leary DD, Wilkinson DG: Eph receptors and ephrins in neural development. Curr Opin Neurobiol. 9: 65-73. 10.1016/S0959-4388(99)80008-7.
35.
Noren NK, Lu M, Freeman AL, Koolpe M, Pasquale EB: Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth. Proc Natl Acad Sci U S A. 2004, 101: 5583-88. 10.1073/pnas.0401381101.CrossRefPubMedPubMedCentral
Metadata
Title
Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma
Authors
Yen-Ching Lee
Janeanne R Perren
Evelyn L Douglas
Michael P Raynor
Maria A Bartley
Peter G Bardy
Sally-Anne Stephenson
Publication date
01-12-2005
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2005
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-5-119

Other articles of this Issue 1/2005

BMC Cancer 1/2005 Go to the issue

Research article

Characterization of the linkage disequilibrium structure and identification of tagging-SNPs in five DNA repair genes

Case report

Zollinger-Ellison syndrome associated with neurofibromatosis type 1: a case report

Research article

Compliance and toxicity of adjuvant CMF in elderly breast cancer patients: a single-center experience

Research article

Novel curcumin- and emodin-related compounds identified by in silico 2D/3D conformer screening induce apoptosis in tumor cells

Research article

Acquisition of anoikis resistance in human osteosarcoma cells does not alter sensitivity to chemotherapeutic agents

Research article

A new survival model for hyperthermic intraperitoneal chemotherapy (HIPEC) in tumor-bearing rats in the treatment of peritoneal carcinomatosis
Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
Developed by: Springer Medicine
Image Credits