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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-09-2019 | Invagination | Basic Science

Mesenchymal cells and fluid flow stimulation synergistically regulate the kinetics of corneal epithelial cells at the air–liquid interface

Authors: Kosuke Kawata, Shigehisa Aoki, Maki Futamata, Mihoko Yamamoto-Rikitake, Isao Nakao, Hiroshi Enaida, Shuji Toda

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 9/2019

Abstract

Purpose

In vivo microenvironments are critical to tissue homeostasis and wound healing, and the cornea is regulated by a specific microenvironment complex that consists of cell–cell interactions, air–liquid interfaces, and fluid flow stimulation. In this study, we aimed to clarify the effects of and the correlations among these three component factors on the cell kinetics of corneal epithelial cells.

Methods

Human corneal epithelial–transformed (HCE–T) cells were cocultured with either primary rat corneal fibroblasts or NIH 3T3 fibroblasts. We employed a double-dish culture method to create an air–liquid interface and a gyratory shaker to create fluid flow stimulation. Morphometric and protein expression analyses were performed for the HCE–T cells.

Results

Both the primary rat fibroblasts and the NIH 3T3 cells promoted HCE–T cell proliferation, and the presence of fluid flow synergistically enhanced this effect and inhibited the apoptosis of HCE–T cells. Moreover, fluid flow enhanced the emergence of myofibroblasts when cocultured with primary rat fibroblasts or NIH 3T3 cells. Extracellular signal-regulated kinase and p38 signaling were regulated either synergistically or independently by both fluid flow and cellular interaction between the HCE–T and NIH 3T3 cells.

Conclusion

The cell–cell interaction and fluid flow stimulation in the air–liquid interface synergistically or independently regulated the behavior of HCE–T cells. Fluid flow accelerated the phenotypic change from corneal fibroblasts and NIH 3T3 cells to myofibroblasts. Elucidation of the multicomponent interplay in this microenvironment will be critical to the homeostasis and regeneration of the cornea and other ocular tissues.

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Title: Mesenchymal cells and fluid flow stimulation synergistically regulate the kinetics of corneal epithelial cells at the air–liquid interface
Authors: Kosuke Kawata
Shigehisa Aoki
Maki Futamata
Mihoko Yamamoto-Rikitake
Isao Nakao
Hiroshi Enaida
Shuji Toda
Publication date: 01-09-2019
Publisher: Springer Berlin Heidelberg
Keyword: Invagination
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 9/2019
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-019-04422-y

At a glance: The STEP trials

Springer Medicine

Mesenchymal cells and fluid flow stimulation synergistically regulate the kinetics of corneal epithelial cells at the air–liquid interface

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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